膜小胞に外来タンパク質を積み込む手法

Kawamoto J., Kurihara T. (2022) Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures. In: Bidmos F., Bossé J., Langford P. (eds) Bacterial Vaccines. Methods in Molecular Biology, vol 2414. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1900-1_12

外部環境に応答して膜小胞生産を制御するセンサータンパク質

ホモログの同定

Yokoyama F, Imai T, Aoki W, Ueda M, Kawamoto J, Kurihara T. Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13. Front Microbiol. 2021 Feb 18;12:629023. doi: 10.3389/fmicb.2021.629023. PMID: 33679653; PMCID: PMC7930318.

膜小胞の膜を構成するリポオリゴ糖の構造決定

Di Guida R, Casillo A, Yokoyama F, Kawamoto J, Kurihara T, Corsaro MM. Detailed Structural Characterization of the Lipooligosaccharide from the Extracellular Membrane Vesicles of Shewanella vesiculosa HM13. Mar Drugs. 2020 Apr 27;18(5):231. doi: 10.3390/md18050231. PMID: 32349432; PMCID: PMC7281004.

膜小胞へのタンパク質輸送に関与する遺伝子クラスターの機能解析

Kamasaka K, Kawamoto J, Chen C, Yokoyama F, Imai T, Ogawa T, Kurihara T. Genetic characterization and functional implications of the gene cluster for selective protein transport to extracellular membrane vesicles of Shewanella vesiculosa HM13. Biochem Biophys Res Commun. 2020 May 28;526(2):525-531. doi: 10.1016/j.bbrc.2020.03.125. Epub 2020 Mar 31. PMID: 32245618.

膜小胞高生産性細菌の細胞表層リポオリゴ糖の構造決定

Casillo A, Di Guida R, Carillo S, Chen C, Kamasaka K, Kawamoto J, Kurihara T, Corsaro MM. Structural Elucidation of a Novel Lipooligosaccharide from the Antarctic Bacterium OMVs Producer Shewanella sp. HM13. Mar Drugs. 2019 Jan 8;17(1):34. doi: 10.3390/md17010034. PMID: 30626008; PMCID: PMC6357163.

膜小胞高生産性細菌の発見、膜小胞の脂質・タンパク質組成解析、膜小胞積荷タンパク質の輸送機構解析

Chen C, Kawamoto J, Kawai S, Tame A, Kato C, Imai T, Kurihara T. Isolation of a Novel Bacterial Strain Capable of Producing Abundant Extracellular Membrane Vesicles Carrying a Single Major Cargo Protein and Analysis of Its Transport Mechanism. Front Microbiol. 2020 Jan 14;10:3001. doi: 10.3389/fmicb.2019.03001. PMID: 32010084; PMCID: PMC6971210.

細菌の膜リン脂質組成が膜小胞生産性に及ぼす影響の解析

Yokoyama F, Kawamoto J, Imai T, Kurihara T. Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition. Extremophiles. 2017 Jul;21(4):723-731. doi: 10.1007/s00792-017-0937-z. Epub 2017 Apr 22. PMID: 28434130.

1. *Cover photo: Shewanella vesiculosa HM13