微生物酵素の機能解析
Microbial Enzymes
細菌による DHA の EPA への変換反応を担うβ-酸化系関連酵素の
同定と機能解析
Ogawa T, Hirose K, Yusuf Y, Kawamoto J, Kurihara T. Bioconversion From Docosahexaenoic Acid to Eicosapentaenoic Acid in the Marine Bacterium Shewanella livingstonensis Ac10. Front Microbiol. 2020 May 26;11:1104. doi: 10.3389/fmicb.2020.01104. PMID: 32528457; PMCID: PMC7264947.
https://pubmed.ncbi.nlm.nih.gov/32528457/
細菌による DHA の EPA への変換反応を担うβ-酸化系初発酵素の
同定と機能解析
Yusuf Y, Ogawa T, Kawamoto J, Kurihara T. Role of acyl-CoA dehydrogenases from Shewanella livingstonensis Ac10 in docosahexaenoic acid conversion. Biochem Biophys Res Commun. 2020 Jul 30;528(3):453-458. doi: 10.1016/j.bbrc.2020.05.185. Epub 2020 Jun 4. PMID: 32507597.
https://pubmed.ncbi.nlm.nih.gov/32507597/
好熱菌の膜リン脂質 sn-2 位にアシル鎖を導入する酵素の
精製と特性解析
Ogawa T, Suwanawat N, Toyotake Y, Watanabe B, Kawamoto J, Kurihara T. Lysophosphatidic acid acyltransferase from the thermophilic bacterium Thermus thermophilus HB8 displays substrate promiscuity. Biosci Biotechnol Biochem. 2020 Sep;84(9):1831-1838. doi: 10.1080/09168451.2020.1771169. Epub 2020 May 26. PMID: 32456605.
https://pubmed.ncbi.nlm.nih.gov/32456605/
大腸菌の膜リン脂質 sn-2 位にアシル鎖を導入する酵素の同定と
べん毛形成への関与の発見
Toyotake Y, Nishiyama M, Yokoyama F, Ogawa T, Kawamoto J, Kurihara T. A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation. Biomolecules. 2020 May 11;10(5):745. doi: 10.3390/biom10050745. PMID: 32403425; PMCID: PMC7277886.
https://pubmed.ncbi.nlm.nih.gov/32403425/
細菌の膜リン脂質 sn-2 位に分岐アシル鎖を導入する酵素の発見
Toyotake Y, Cho HN, Kawamoto J, Kurihara T. A novel 1-acyl-sn-glycerol-3-phosphate O-acyltransferase homolog for the synthesis of membrane phospholipids with a branched-chain fatty acyl group in Shewanella livingstonensis Ac10. Biochem Biophys Res Commun. 2018 Jun 7;500(3):704-709. doi: 10.1016/j.bbrc.2018.04.140. Epub 2018 Apr 23. Erratum in: Biochem Biophys Res Commun. 2019 Feb 19;509(4):1053. Erratum in: Biochem Biophys Res Commun. 2020 May 14;525(4):1109. PMID: 29678574.
https://pubmed.ncbi.nlm.nih.gov/29678574/
細菌の膜リン脂質sn-2位にEPAを導入する酵素の精製と特性解析
Ogawa T, Tanaka A, Kawamoto J, Kurihara T. Purification and characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase with a substrate preference for polyunsaturated fatty acyl donors from the eicosapentaenoic acid-producing bacterium Shewanella livingstonensis Ac10. J Biochem. 2018 Jul 1;164(1):33-39. doi: 10.1093/jb/mvy025. PMID: 29415144.
