細菌による DHA の EPA への変換反応を担うβ-酸化系関連酵素の

同定と機能解析

Ogawa T, Hirose K, Yusuf Y, Kawamoto J, Kurihara T. Bioconversion From Docosahexaenoic Acid to Eicosapentaenoic Acid in the Marine Bacterium Shewanella livingstonensis Ac10. Front Microbiol. 2020 May 26;11:1104. doi: 10.3389/fmicb.2020.01104. PMID: 32528457; PMCID: PMC7264947.

https://pubmed.ncbi.nlm.nih.gov/32528457/

細菌による DHA の EPA への変換反応を担うβ-酸化系初発酵素の

同定と機能解析

Yusuf Y, Ogawa T, Kawamoto J, Kurihara T. Role of acyl-CoA dehydrogenases from Shewanella livingstonensis Ac10 in docosahexaenoic acid conversion. Biochem Biophys Res Commun. 2020 Jul 30;528(3):453-458. doi: 10.1016/j.bbrc.2020.05.185. Epub 2020 Jun 4. PMID: 32507597.

https://pubmed.ncbi.nlm.nih.gov/32507597/

好熱菌の膜リン脂質 sn-2 位にアシル鎖を導入する酵素の

精製と特性解析

Ogawa T, Suwanawat N, Toyotake Y, Watanabe B, Kawamoto J, Kurihara T. Lysophosphatidic acid acyltransferase from the thermophilic bacterium Thermus thermophilus HB8 displays substrate promiscuity. Biosci Biotechnol Biochem. 2020 Sep;84(9):1831-1838. doi: 10.1080/09168451.2020.1771169. Epub 2020 May 26. PMID: 32456605.

https://pubmed.ncbi.nlm.nih.gov/32456605/

大腸菌の膜リン脂質 sn-2 位にアシル鎖を導入する酵素の同定と

べん毛形成への関与の発見

Toyotake Y, Nishiyama M, Yokoyama F, Ogawa T, Kawamoto J, Kurihara T. A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation. Biomolecules. 2020 May 11;10(5):745. doi: 10.3390/biom10050745. PMID: 32403425; PMCID: PMC7277886.

https://pubmed.ncbi.nlm.nih.gov/32403425/

細菌の膜リン脂質 sn-2 位に分岐アシル鎖を導入する酵素の発見

Toyotake Y, Cho HN, Kawamoto J, Kurihara T. A novel 1-acyl-sn-glycerol-3-phosphate O-acyltransferase homolog for the synthesis of membrane phospholipids with a branched-chain fatty acyl group in Shewanella livingstonensis Ac10. Biochem Biophys Res Commun. 2018 Jun 7;500(3):704-709. doi: 10.1016/j.bbrc.2018.04.140. Epub 2018 Apr 23. Erratum in: Biochem Biophys Res Commun. 2019 Feb 19;509(4):1053. Erratum in: Biochem Biophys Res Commun. 2020 May 14;525(4):1109. PMID: 29678574.

https://pubmed.ncbi.nlm.nih.gov/29678574/

細菌の膜リン脂質sn-2位にEPAを導入する酵素の精製と特性解析

Ogawa T, Tanaka A, Kawamoto J, Kurihara T. Purification and characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase with a substrate preference for polyunsaturated fatty acyl donors from the eicosapentaenoic acid-producing bacterium Shewanella livingstonensis Ac10. J Biochem. 2018 Jul 1;164(1):33-39. doi: 10.1093/jb/mvy025. PMID: 29415144.

https://pubmed.ncbi.nlm.nih.gov/29415144/

EPAの細胞内動態の解析に有用なエチニル基導入型

EPAアナログの開発

Tokunaga T, Watanabe B, Sato S, Kawamoto J, Kurihara T. Synthesis and Functional Assessment of a Novel Fatty Acid Probe, ω-Ethynyl Eicosapentaenoic Acid Analog, to Analyze the in Vivo Behavior of Eicosapentaenoic Acid. Bioconjug Chem. 2017 Aug 16;28(8):2077-2085. doi: 10.1021/acs.bioconjchem.7b00235. Epub 2017 Jul 24. PMID: 28682621.

https://pubmed.ncbi.nlm.nih.gov/28682621/

EPA含有リン脂質のシャペロン機能の発見

Dai XZ, Kawamoto J, Sato SB, Esaki N, Kurihara T. Eicosapentaenoic acid facilitates the folding of an outer membrane protein of the psychrotrophic bacterium, Shewanella livingstonensis Ac10. Biochem Biophys Res Commun. 2012 Aug 24;425(2):363-7. doi: 10.1016/j.bbrc.2012.07.097. Epub 2012 Jul 25. PMID: 22842563.

https://pubmed.ncbi.nlm.nih.gov/22842563/

多価不飽和炭化水素鎖を有するリン脂質が細菌の細胞分裂部位に

集積することの発見

Sato S, Kawamoto J, Sato SB, Watanabe B, Hiratake J, Esaki N, Kurihara T. Occurrence of a bacterial membrane microdomain at the cell division site enriched in phospholipids with polyunsaturated hydrocarbon chains. J Biol Chem. 2012 Jul 13;287(29):24113-21. doi: 10.1074/jbc.M111.318311. Epub 2012 May 30. PMID: 22648406; PMCID: PMC3397838.

https://pubmed.ncbi.nlm.nih.gov/22648406/