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**''Create New World of Bioactive Synthetic Molecules''&br;''New ways to use, New Shapes, New sizes.'' [#m6455066]
As biological processes all stem from chemical events, it should be possible to understand or manipulate biological events by using chemistry. As chemical biologists our challenge is to discover or design unique organic molecules, 'super tools' that modulate fundamental processes in human cells. Using these 21st century super tools we can explore complex cellular events. Our job is creation: designing a new world of bioactive synthetic molecules, with unique and novel uses, forms and sizes. The future for small-molecule applications is open before us, in a range of fields, including future drug discovery and cell therapy. Our 21st century technology and skills will revolutionize the fight against the most difficult diseases and medical challenges of the age.
**''ケミカルバイオロジー～化学を起点とした生物学'' [#c193215c]
ケミカルバイオロジーとは、化学を起点とした生物学です。生命の営みはせんじつめれば化学反応でできています。逆に化学を使って生命現象を理解したり、操ることができるはず。私たちの研究室では、生き物やヒト細胞にユニークな効果を及ぼす独自な有機化合物を見つける、もしくはデザインし、それらを道具として生命現象を探究・操作してきました。生物の仕組みは複雑ですが、有機化合物を起爆剤として用いることで、新たな切り口で生物を研究したり操作することができます。私たちの大きな研究目標は、生理活性合成化合物の新しい世界を切り開くことです。生理活性化合物の新しい利用法、新しいカタチをした生理活性化合物、新しいサイズの生理活性化合物など、アイデアを与えることができればと思うのです。こういったアイデアは、未来の創薬の考え方や化合物の細胞治療への利用などに結びつくはずです。
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*Selected Publications [#jd61e510]
**''Small-Molecule Drug Repurposing for Counteracting Phototoxic A2E Aggregation'' [#p6d61129]
*Selected Publications [#w3c77657]
**''Identification of a Self-Assembling Small-Molecule Cancer Vaccine Adjuvant with an Improved Toxicity Profile'' [#m8dc334a]
***Perron, A., Mandal, S., Chuba, T., Mao, D., Singh, V., Noda, N., Tan, R., Vu, H., Abo, M., Uesugi, M. [#qe2ca6a8]
***Zhuo, S., Noda, N., Hioki, K., Jin, S., Hayashi, T., Hiraga, K., Momose, H., Li, W., Zhao, L., Mizukami, T., Ishii, K., Li, Y., Uesugi, M. [#jb8dc4b7]
'''''ACS Chem. Biol.''''' 18(10), 2170–2175 (2023)&br;
https://doi.org/10.1021/acschembio.3c00339
#ref((2023) Small-Molecule Drug Repurposing for Counteracting Phototoxic A2E Aggregation.gif,nolink,70%)
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**''Identification of a Self-Assembling Small-Molecule Cancer Vaccine Adjuvant with an Improved Toxicity Profile'' [#p6d61129]
***Zhuo, S., Noda, N., Hioki, K., Jin, S., Hayashi, T., Hiraga, K., Momose, H., Li, W., Zhao, L., Mizukami, T., Ishii, K., Li, Y., Uesugi, M.[#b4ffcffa]
'''''J. Med. Chem.''''' 66(18), 13266–13279 (2023)&br;
https://doi.org/10.1021/acs.jmedchem.3c01252
#ref((2023) Identification of a Self-Assembling Small-Molecule Cancer Vaccine Adjuvant with an Improved Toxicity Profile.gif,nolink,80%)
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**''Chemoproteomic Identification of Blue-Light-Damaged Proteins''[#j4323c7d]
**''Chemoproteomic Identification of Blue-Light-Damaged Proteins'' [#k221a8c0]
***Toh, K., Nishio, K., Nakagawa, R., Egoshi, S., Abo, M., Perron, A., Sato, S., Okumura, N., Koizumi, N., Dodo, K., Sodeoka, M., Uesugi, M.[#b4ffcffa]
***Toh, K., Nishio, K., Nakagawa, R., Egoshi, S., Abo, M., Perron, A., Sato, S., Okumura, N., Koizumi, N., Dodo, K., Sodeoka, M., Uesugi, M. [#dec91b93]
'''''J. Am. Chem. Soc. ''''' 144(44), 20171–20176 (2022)&br;
https://doi.org/10.1021/jacs.2c07180
#ref((2022) Chemoproteomic Identification of Blue-Light-Damaged Proteins.jpg,nolink,80%)
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**''Self-assembling small-molecule adjuvants as antigen nano-carriers''[#j4323c7d]
**''Magnetic Control of Cells by Chemical Fabrication of Melanin'' [#r8fce3fc]
***Jin, S., Zhuo, S., Takemoto, Y., Li, Y., Uesugi, M.[#b4ffcffa]
***Nishio, K., Toh, K., Perron, A., Goto, M., Abo, M., Shimakawa, Y., Uesugi, M. [#aa4551cf]
'''''Chem. Comm. ''''' 58, 12228-12231 (2022)&br;
https://doi.org/10.1039/D2CC05016A
#ref((2022) Self-assembling small-molecule adjuvants as antigen nano-carriers.png,nolink,65%)
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**''Magnetic Control of Cells by Chemical Fabrication of Melanin''[#j4323c7d]
***Nishio, K., Toh, K., Perron, A., Goto, M., Abo, M., Shimakawa, Y., Uesugi, M.[#b4ffcffa]
'''''J. Am. Chem. Soc.''''' 144(37), 16720–16725 (2022)&br;
https://doi.org/10.1021/jacs.2c06555
#ref((2022)Magnetic Control of Cells by Chemical Fabrication of Melanin.jpg,nolink,80%)
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**''Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells''[#j4323c7d]
**''Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells'' [#e8a14d91]
***Ado, G., Noda, N., Vu, H., Perron, A., Mahapatra, A., Arista, K., Yoshimura, H., Packwood, D., Ishidate, F., Sato, S., Ozawa, T., Uesugi, M.[#b4ffcffa]
***Ado, G., Noda, N., Vu, H., Perron, A., Mahapatra, A., Arista, K., Yoshimura, H., Packwood, D., Ishidate, F., Sato, S., Ozawa, T., Uesugi, M. [#w0d89575]
'''''Chemical Science''''' 13, 5760-5766 (2022)&br;
'''''Chemical Science''''' 13, 5760-5766 (2022)&br;
https://doi.org/10.1039/D1SC07151C
#ref((2022) Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells.jpg,nolink,110%)
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**''Glucose as a Protein-Condensing Cellular Solute'' [#n5f0b984]
**''Glucose as a Protein-Condensing Cellular Solute''[#j4323c7d]
***Noda, N., Jung, Y., Ado, G., Mizuhata, Y., Higuchi, M., Ogawa, T., Ishidate, F., Sato, S., Kurata, H., Tokitoh, N., Uesugi, M. [#x9ab51b2]
***Noda, N., Jung, Y., Ado, G., Mizuhata, Y., Higuchi, M., Ogawa, T., Ishidate, F., Sato, S., Kurata, H., Tokitoh, N., Uesugi, M.[#b4ffcffa]
'''''ACS Chem. Biol.''''' 17(3), 567–575 (2022)&br;
https://doi.org/10.1021/acschembio.1c00849
#ref((2022)Glucose as a Protein-Condensing Cellular Solute.gif,nolink,80%)
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**''Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite.'' [#j8a5c49a]
**''Discovery of Non-Cysteine-Targeting Covalent Inhibitors by Activity-Based Proteomic Screening with a Cysteine-Reactive Probe''[#j4323c7d]
***Mendoza, A., Takemoto, Y., Cruzado, K., Masoud, S., Nagata, A., Tantipanjaporn, A., Okuda, S., Kawagoe, F., Sakamoto, R., Odagi, M., Mototani, S., Togashi, M., Kawatani, M., Aono, H., Osada, H., Nakagawa, H., Higashi, T., Kittaka, A., Nagasawa, K., Uesugi, M. [#l9293f8b]
***Jung, Y., Noda, N., Takaya, J., Abo, M., Toh, K., Tajiri, K., Cui, C., Zhou, L., Sato, S., Uesugi, M.[#b4ffcffa]
'''''ACS Chem. Biol.''''' 17(2), 340–347 (2022)&br;
https://doi.org/10.1021/acschembio.1c00824
#ref((2022)Discovery of Non-Cysteine-Targeting Covalent Inhibitors.gif,nolink,80%)
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**''Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite.''[#j4323c7d]
***Mendoza, A., Takemoto, Y., Cruzado, K., Masoud, S., Nagata, A., Tantipanjaporn, A., Okuda, S., Kawagoe, F., Sakamoto, R., Odagi, M., Mototani, S., Togashi, M., Kawatani, M., Aono, H., Osada, H., Nakagawa, H., Higashi, T., Kittaka, A., Nagasawa, K., Uesugi, M.[#b4ffcffa]
'''''Cell Chemical Biology''''' 29(4), 660-669 (2022)&br;
'''''Cell Chemical Biology''''' 29(4), 660-669 (2022)&br;
https://doi.org/10.1016/j.chembiol.2021.08.008
#ref((2021) Controlled lipid β-oxidation and carnitine biosynthesis by a vitamin D metabolite.jpg,nolink,100%)
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**''Chemical Genetics Reveals a Role of Squalene Synthase in TGFβ Signaling and Cardiomyogenesis'' [#j4323c7d]
***Takemoto, Y., Kadota, S., Minami, I., Otsuka, S., Okuda, S., Abo, M., Punzalan, L., Shen, Y., Shiba, Y., Uesugi, M.[#b4ffcffa]
**''Chemical Genetics Reveals a Role of Squalene Synthase in TGFβ Signaling and Cardiomyogenesis'' [#w61fc138]
***Takemoto, Y., Kadota, S., Minami, I., Otsuka, S., Okuda, S., Abo, M., Punzalan, L., Shen, Y., Shiba, Y., Uesugi, M. [#b3ee4b1c]
'''''Angew. Chem. Int. Ed.''''' 60(40), 21824-21831 (2021)&br;
https://doi.org/10.1002/anie.202100523
#ref((2021) Chemical Genetics Reveals a Role of Squalene Synthase in TGFβ Signaling and Cardiomyogenesis.png,nolink,25%)
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**''Non-genetic cell-surface modification with a self-assembling molecular glue.'' [#j4323c7d]
***Hakariya, H., Takashima, I., Takemoto, M., Noda, N., Sato, S., Uesugi, M. [#b4ffcffa]
**''Discovery of Self‐Assembling Small Molecules as Vaccine Adjuvants.'' [#q3222975]
***Jin, S., Vu, H., Hioki, K., Noda, N., Yoshida, H., Shimane, T., Ishizuka, S., Takashima, I., Mizuhata, Y., Pe, K., Ogawa, T., Nishimura, N., Packwood, D., Tokitoh, N., Kurata, H., Yamasaki, S., Ishii, K., Uesugi, M. [#s1a8c1f9]
'''''Chem. Comm.''''' 57(12), 1470-1473 (2021)&br;
https://doi.org/10.1039/d0cc07171d
#ref(research41 (2021).gif,nolink,100%)
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**''Discovery of Self‐Assembling Small Molecules as Vaccine Adjuvants.'' [#j4323c7d]
***Jin, S., Vu, H., Hioki, K., Noda, N., Yoshida, H., Shimane, T., Ishizuka, S., Takashima, I., Mizuhata, Y., Pe, K., Ogawa, T., Nishimura, N., Packwood, D., Tokitoh, N., Kurata, H., Yamasaki, S., Ishii, K., Uesugi, M. [#b4ffcffa]
'''''Angew. Chem. Int. Ed.''''' 60(2), 961-969 (2021)&br;
https://doi.org/10.1002/anie.202011604
#ref((2020) Discovery of Self‐Assembling Small Molecules as Vaccine Adjuvants.png,nolink,17%)
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**''Chemoproteomic Profiling of a Pharmacophore-Focused Chemical Library.'' [#j4323c7d]
***Punzalan, L., Jiang, L., Mao, D., Mahapatra, A., Sato, S., Takemoto, Y., Tsujimura, M., Kusamori, K., Nishikawa, M., Zhou, L., Uesugi, M. [#b4ffcffa]
**''Chemoproteomic Profiling of a Pharmacophore-Focused Chemical Library.'' [#o173681e]
***Punzalan, L., Jiang, L., Mao, D., Mahapatra, A., Sato, S., Takemoto, Y., Tsujimura, M., Kusamori, K., Nishikawa, M., Zhou, L., Uesugi, M. [#ib6d0123]
'''''Cell Chemical Biology''''' 27(6), 708–718 (2020)&br;
https://doi.org/10.1016/j.chembiol.2020.04.007
#ref(research2020-2.png,nolink,70%)
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**''Discovery of a Small-Molecule-Dependent Photolytic Peptide.'' [#j4323c7d]
***Takemoto, Y., Mao, Di., Punzalan, L., Götze, S., Sato, S., Uesugi, M. [#b4ffcffa]
**''Discovery of a Small-Molecule-Dependent Photolytic Peptide.'' [#zdff2121]
***Takemoto, Y., Mao, Di., Punzalan, L., Götze, S., Sato, S., Uesugi, M. [#ce48d0e2]
'''''J. Am. Chem. Soc.''''' 142(3), 1142-1146 (2020)&br;
https://doi.org/10.1021/jacs.9b09178
#ref(research38 (2020).jpg,nolink,30%)
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**''Nutrient-Based Chemical Library as a Source of Energy Metabolism Modulators.'' [#jd97243e]
***Furuta,T., Mizukami, Y., Asano, L., Kotake, K., Ziegler, S., Yoshida, H., Watanabe, M., Sato, S., Waldmann, H., Nishikawa, M., Uesugi, M. [#nd081d05]
**''Synthetic Chemical Probes That Dissect Vitamin D Activities.'' [#j4323c7d]
***Nagata, A., Akagi, Y., Asano, L., Kotake, K., Kawagoe, F., Mendoza, A., Masoud, S., Usuda, K., Yasui, K., Takemoto, Y., Kittaka, A., Nagasawa, K., Uesugi, M. [#b4ffcffa]
'''''ACS Chem. Biol.''''' 14(12), 2851–2858 (2019)&br;
https://doi.org/10.1021/acschembio.9b00718
#ref(research37 (2019).gif,nolink,60%)
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**''Nutrient-Based Chemical Library as a Source of Energy Metabolism Modulators.'' [#j4323c7d]
***Furuta,T., Mizukami, Y., Asano, L., Kotake, K., Ziegler, S., Yoshida, H., Watanabe, M., Sato, S., Waldmann, H., Nishikawa, M., Uesugi, M. [#b4ffcffa]
'''''ACS Chem. Biol.''''' 14(9), 1860-1865 (2019)&br;
https://doi.org/10.1021/acschembio.9b00444
#ref(Nutrient-Based Chemical Library as a Source of Energy Metabolism Modulators.png,nolink,50%)
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**''Vitamin D metabolite, 25-Hydroxyvitamin D, regulates lipid metabolism by inducing degradation of SREBP/SCAP.'' [#ve7ac7a8]
***Asano, L., Watanabe, M., Ryoden, Y., Usuda, K., Yamaguchi, T., Khambu, B., Takashima, M., Sato, S., Sakai, J., Nagasawa, K., Uesugi, M. [#g4a27693]
**''Multifunctionalization of cells with a self-assembling molecule to enhance cell engraftment.'' [#j4323c7d]
***Takashima, I., Kusamori, K., Hakariya, H., Takashima, M., Vu, TH., Mizukami, Y., Noda, N., Takayama, Y., Katsuda, Y., Sato, S., Takakura, Y., Nishikawa, M., Uesugi, M. [#b4ffcffa]
'''''ACS Chem. Biol., '''''14(4), 775–783 (2019)&br;
https://doi.org/10.1021/acschembio.9b00109
#ref(cb-2019-00109g_0007.jpg,nolink,20%)
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**''Small-molecule screening yields a compound that inhibits the cancer-associated transcription factor Hes1 via the PHB2 chaperone.'' [#j4323c7d]
***Perron, A., Nishikawa, Y., Iwata, J., Shimojo, H., Takaya, J., Kobayashi, K., Imayoshi, I., Mbenza, N. M,, Takenoya, M., Kageyama, R., Kodama, Y., Uesugi, M. [#b4ffcffa]
'''''J. Biol. Chem.''''' 293(21), 8285-8294 (2018)&br;
https://doi.org/10.1074/jbc.RA118.002316
#ref(zbc0181885870001[1].jpg,nolink,50%)
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**''Chemical decontamination of iPS cell-derived neural cell mixtures. '' [#j4323c7d]
***Mao, D., Chung, X. K. W., Andoh-Noda, T., Qin, Y., Sato, S., Takemoto, Y., Akamatsu, W., Okano, H., Uesugi, M. [#b4ffcffa]
'''''Chem. Commun.''''' 54, 1355-1358 (2018)&br;
https://doi.org/10.1039/C7CC08686E
#ref(dimao.gif,nolink)
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**''Vitamin D metabolite, 25-Hydroxyvitamin D, regulates lipid metabolism by inducing degradation of SREBP/SCAP.'' [#mef7b73d]
***Asano, L., Watanabe, M., Ryoden, Y., Usuda, K., Yamaguchi, T., Khambu, B., Takashima, M., Sato, S., Sakai, J., Nagasawa, K., Uesugi, M. [#hc2fdbc9]
'''''Cell Chem Biol.''''' 24, 207-217 (2017)&br;
https://doi.org/10.1016/j.chembiol.2016.12.017
#ref(research01.jpg,nolink,375x375)
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**''A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures.'' [#j4323c7d]
***Mao, D., Ando, S., Sato, S., Qin, Y., Hirata, N., Katsuda, Y., Kawase, E., Kuo, T.F., Minami, I., Shiba, Y., Ueda, K., Nakatsuji, N., Uesugi, M. [#b4ffcffa]
**''A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures.'' [#hfe67f31]
***Mao, D., Ando, S., Sato, S., Qin, Y., Hirata, N., Katsuda, Y., Kawase, E., Kuo, T.F., Minami, I., Shiba, Y., Ueda, K., Nakatsuji, N., Uesugi, M. [#xb045a18]
'''''Angew. Chem. Int. Ed.''''' 56(7), 1765-1770 (2017)&br;
https://doi.org/10.1002/anie.201610284
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**''A small molecule that represses translation of G-quadruplex-containing mRNA.'' [#u8c60a82]
***Katsuda, Y., Sato, S., Asano, L., Morimura, Y., Furuta, T., Sugiyama, H., Hagihara, M., Uesugi, M. [#be33e28d]
**''A small molecule that represses translation of G-quadruplex-containing mRNA.'' [#t13dde57]
***Katsuda, Y., Sato, S., Asano, L., Morimura, Y., Furuta, T., Sugiyama, H., Hagihara, M., Uesugi, M. [#l1ed95c2]
'''''J. Am. Chem. Soc.''''' 138, 9037-9040 (2016)&br;
https://doi.org/10.1021/jacs.6b04506
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**''A potent and site-selective agonist of TRPA1.'' [#eb6719bd]
***Takaya, J., Mio, K., Shiraishi, T., Kurokawa, T., Otsuka, S., Mori, Y., Uesugi, M. [#z5723179]
**''A potent and site-selective agonist of TRPA1.'' [#b350c1e2]
***Takaya, J., Mio, K., Shiraishi, T., Kurokawa, T., Otsuka, S., Mori, Y., Uesugi, M. [#n3c774d9]
'''''J. Am. Chem. Soc.''''' 137, 15859−15864 (2015)&br;
https://doi.org/10.1021/jacs.5b10162
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**''Intracellular generation of a diterpene-peptide conjugate that inhibits 14-3-3-mediated interactions.'' [#u3589e6e]
***Parvatkar, P., Kato, N., Uesugi, M., Sato, S., Ohkanda, J. [#afa267b4]
**''Intracellular generation of a diterpene-peptide conjugate that inhibits 14-3-3-mediated interactions.'' [#t619e66d]
***Parvatkar, P., Kato, N., Uesugi, M., Sato, S., Ohkanda, J. [#ea25bcb6]
'''''J. Am. Chem. Soc.''''' 137, 15624-15627 (2015)&br;
https://doi.org/10.1021/jacs.5b09817
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**''Live-cell imaging of endogenous mRNAs with a small molecule.'' [#m6b741fb]
***Sato, S., Watanabe, M., Katsuda, Y., Murata, A., Wang, D. O., Uesugi M. [#w35ae47a]
**''Live-cell imaging of endogenous mRNAs with a small molecule.'' [#u1a02109]
***Sato, S., Watanabe, M., Katsuda, Y., Murata, A., Wang, D. O., Uesugi M. [#z7a6f4dc]
'''''Angew. Chem. Int. Ed.''''' 54(6), 1855-1858 (2015)&br;
https://doi.org/10.1002/anie.201410339
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**''Synthetic molecules that protect cells from anoikis and their use in cell transplantation.'' [#x4f90b90]
***Frisco-Cabanos, H.L., Watanabe, M., Okumura, N., Kusamori, K., Takemoto, N., Takaya, J., Sato, S., Yamazoe, S., Takakura, Y., Kinoshita, S., Nishikawa, M., Koizumi, N., Uesugi, M. [#c589fd92]
**''Synthetic molecules that protect cells from anoikis and their use in cell transplantation.'' [#zdf424c8]
***Frisco-Cabanos, H.L., Watanabe, M., Okumura, N., Kusamori, K., Takemoto, N., Takaya, J., Sato, S., Yamazoe, S., Takakura, Y., Kinoshita, S., Nishikawa, M., Koizumi, N., Uesugi, M. [#i25dc0b6]
'''''Angew. Chem. Int. Ed.''''' 53(42), 11208-11213 (2014)&br;
https://doi.org/10.1002/anie.201405829
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**''Selective elimination of human pluripotent stem cells by a marine natural product derivative.'' [#ra8e9143]
***Kuo, T.F., Mao, D., Hirata, N., Khambu, B., Kimura, Y., Kawase, E., Shimogawa, H., Ojika, M., Nakatsuji, N., Ueda, K., Uesugi, M. [#hd585ec0]
**''Selective elimination of human pluripotent stem cells by a marine natural product derivative.'' [#q9dadb27]
***Kuo, T.F., Mao, D., Hirata, N., Khambu, B., Kimura, Y., Kawase, E., Shimogawa, H., Ojika, M., Nakatsuji, N., Ueda, K., Uesugi, M. [#g4b385e0]
'''''J. Am. Chem. Soc.''''' 136 (28), 9798-9801 (2014)&br;
https://doi.org/10.1021/ja501795c
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**''A chemical probe that labels human pluripotent stem cells.'' [#u5812274]
***Hirata, N., Nakagawa, M., Fujibayashi, Y., Yamauchi, K., Murata, A., Minami, I., Tomioka, M., Kondo, T., Kuo, T.F., Endo, H., Inoue, H., Sato, S., Ando, S., Kawazoe, Y., Aiba, K., Nagata, K., Kawase, E., Chang, Y.T., Suemori, H., Eto, K., Nakauchi, H., Yamanaka, S., Nakatsuji, N., Ueda, K., Uesugi, M. [#edc8f56f]
**''A chemical probe that labels human pluripotent stem cells.'' [#dc4d680e]
***Hirata, N., Nakagawa, M., Fujibayashi, Y., Yamauchi, K., Murata, A., Minami, I., Tomioka, M., Kondo, T., Kuo, T.F., Endo, H., Inoue, H., Sato, S., Ando, S., Kawazoe, Y., Aiba, K., Nagata, K., Kawase, E., Chang, Y.T., Suemori, H., Eto, K., Nakauchi, H., Yamanaka, S., Nakatsuji, N., Ueda, K., Uesugi, M. [#l98608c3]
'''''Cell Rep''''' 6., 1165–1174 (2014)&br;
https://doi.org/10.1016/j.celrep.2014.02.006
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**''VMAT2 identified as a regulator of late-stage beta cell differentiation.'' [#x9a021aa]
***Sakano, D., Shiraki, N., Kikawa, K., Yamazoe, T., Kataoka, M., Umeda, K., Araki, K., Mao, D., Matsumoto, S., Nakagata, N., Andersson, O., Stainier, D., Endo, F., Kume, K., Uesugi, M., Kume, S. [#d0888c8e]
**''VMAT2 identified as a regulator of late-stage beta cell differentiation.'' [#j8d94e63]
***Sakano, D., Shiraki, N., Kikawa, K., Yamazoe, T., Kataoka, M., Umeda, K., Araki, K., Mao, D., Matsumoto, S., Nakagata, N., Andersson, O., Stainier, D., Endo, F., Kume, K., Uesugi, M., Kume, S. [#mbcd4d87]
'''''Nat. Chem. Biol.''''' 10, 141-148 (2014)&br;
https://doi.org/10.1038/nchembio.1410
#ref(research10.png,nolink,350x201)
''&br;''
''&br;''
**''Inhibition of microtubule assembly by a complex of actin and antitumor macrolide aplyronine A.'' [#o5ac5d86]
***Kita, M., Hirayama, Y., Yoneda, K., Yamagishi, K., Chinen, T., Usui, T., Sumiya, E., Uesugi, M., Kigoshi, H. [#ucba1662]
**''Inhibition of microtubule assembly by a complex of actin and antitumor macrolide aplyronine A.'' [#ba9252d8]
***Kita, M., Hirayama, Y., Yoneda, K., Yamagishi, K., Chinen, T., Usui, T., Sumiya, E., Uesugi, M., Kigoshi, H. [#i1b0a3c1]
'''''J. Am. Chem. Soc.''''' 135 (48), 18089-18095 (2013)&br;
https://doi.org/10.1021/ja406580w
#ref(research11.gif,nolink,350x150)
''&br;''
''&br;''
**''Small molecule-induced clustering of heparan sulfate promotes cell adhesion.'' [#n2fec12e]
***Takemoto, N., Suehara, T., Frisco, H., Sato, S., Sezaki, T., Kusamori, K., Kawazoe, Y., Park, S., Yamazoe, S., Mizuhata, Y., Inoue, R., Miller, G., Hansen, S., Jayson, G., Gardiner, J., Kanaya, T., Tokitoh, N., Ueda, K., Takakura, Y., Kioka, N., Nishikawa, M., Uesugi, M. [#sc072b75]
**''Small molecule-induced clustering of heparan sulfate promotes cell adhesion.'' [#u41ca947]
***Takemoto, N., Suehara, T., Frisco, H., Sato, S., Sezaki, T., Kusamori, K., Kawazoe, Y., Park, S., Yamazoe, S., Mizuhata, Y., Inoue, R., Miller, G., Hansen, S., Jayson, G., Gardiner, J., Kanaya, T., Tokitoh, N., Ueda, K., Takakura, Y., Kioka, N., Nishikawa, M., Uesugi, M. [#f4e47782]
'''''J. Am. Chem. Soc.''''' 135 (30), 11032-11039 (2013)&br;
https://doi.org/10.1021/ja4018682
#ref(research12.gif,nolink,350x118)
''&br;''
''&br;''
**''A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined cytokine- and xeno-free conditions.'' [#p341b5f4]
***Minami, I., Yamada, K., Otsuji, T.G., Yamamoto, T., Shen, Y., Otsuka, S., Kadota, S., Morone, N., Barve, M., Asai, Y., Tenkova-Heuser, T., Heuser, J. E., Uesugi, M., Aiba, K., Nakatsuji, N. [#x542b96d]
**''A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined cytokine- and xeno-free conditions.'' [#zda898c1]
***Minami, I., Yamada, K., Otsuji, T.G., Yamamoto, T., Shen, Y., Otsuka, S., Kadota, S., Morone, N., Barve, M., Asai, Y., Tenkova-Heuser, T., Heuser, J. E., Uesugi, M., Aiba, K., Nakatsuji, N. [#m5f978b2]
'''''Cell Rep.''''' (5), 1448-1460 (2012)&br;
https://doi.org/10.1016/j.celrep.2012.09.015
#ref(research13.jpg,nolink,375x375)
''&br;''
''&br;''
**''Synthesis and evaluation of diarylthiazole derivatives that inhibit activation of sterol regulatory element-binding proteins.'' [#e4f9ebf8]
***Kamisuki, S., Shirakawa T., Kugimiya, A., Abu-Elheiga, L., Choo, H.-Y., Yamada, K., Shimogawa, H., Wakil, S. J., Uesugi, M. [#cd345c99]
**''Synthesis and evaluation of diarylthiazole derivatives that inhibit activation of sterol regulatory element-binding proteins.'' [#w2a8e467]
***Kamisuki, S., Shirakawa T., Kugimiya, A., Abu-Elheiga, L., Choo, H.-Y., Yamada, K., Shimogawa, H., Wakil, S. J., Uesugi, M. [#b2f823d5]
'''''J. Med. Chem.''''' 54(13), 4923-4927 (2011)&br;
https://doi.org/10.1021/jm200304y
#ref(research14.gif,nolink,350x272)
''&br;''
''&br;''
**''A mitochondrial surface-specific fluorescent probe activated by bioconversion.'' [#qe1760c2]
***Kawazoe, Y., Shimogawa, H., Sato, A., Uesugi, M. [#l92a873a]
**''A mitochondrial surface-specific fluorescent probe activated by bioconversion.'' [#r2134ba9]
***Kawazoe, Y., Shimogawa, H., Sato, A., Uesugi, M. [#s1481141]
'''''Angew. Chem. Int. Ed.''''' 50(24), 5478-5481 (2011)&br;
https://doi.org/10.1002/anie.201100935
#ref(research15.png,nolink,600x179)
''&br;''
''&br;''
**''Small-molecule fluorescent probes for specific RNA targets.'' [#y9394fa3]
***Murata, A., Sato, S., Kawazoe, Y., Uesugi, M. [#q972387f]
**''Marine natural product aurilide activates the OPA1-mediated apoptosis by binding to prohibitin.'' [#me9ba18b]
***Sato, S. Murata, A., Orihara, T., Shirakawa, T., Suenaga, K., Kigoshi, H., Uesugi, M. [#q2fa4b67]
'''''Chem. Comm.''''' 47(16), 4712-4714 (2011)&br;
https://doi.org/10.1039/C1CC10393H
#ref(research16.png,nolink,350x145)
''&br;''
''&br;''
**''Cell-morphology profiling of a natural product library identifies bisebromoamide and miuraenamide A as actin-filament stabilizers.'' [#a322d81f]
***Sumiya, E., Shimogawa, H., Sasaki, H., Tsutsumi, M., Yoshita, K., Ojika, M., Suenaga, K., Uesugi, M. [#vd31236c]
'''''ACS Chem. Biol.''''' 6(5), 425-431 (2011)&br;
https://doi.org/10.1021/cb1003459
#ref(research17.gif,nolink,350x182)
''&br;''
''&br;''
**''Deactivation of STAT6 through Serine 707 phosphorylation by JNK.'' [#e60dac41]
***Shirakawa, T. Kawazoe, Y., Tsujikawa, T., Jung, D., Sato, S., Uesugi, M. [#q17101d6]
'''''J. Biol. Chem.''''' 286, 4003-4010 (2011)&br;
https://doi.org/10.1074/jbc.M110.168435
#ref(research18.jpg,nolink,350x230)
''&br;''
''&br;''
**''Marine natural product aurilide activates the OPA1-mediated apoptosis by binding to prohibitin.'' [#ld7a9ead]
***Sato, S. Murata, A., Orihara, T., Shirakawa, T., Suenaga, K., Kigoshi, H., Uesugi, M. [#g62a3d02]
'''''Chem. Biol.''''' 18 (1), 131-139 (2011)&br;
https://doi.org/10.1016/j.chembiol.2010.10.017
#ref(research19.png,nolink,350x261)
''&br;''
''&br;''
**''Biochemical target isolation for novices: Affinity-based strategies. (Review)'' [#m8649b3a]
***Sato, S. Murata, A., Shirakawa, T., Uesugi, M. [#re6a809c]
**''A small molecule that blocks fat synthesis by inhibiting the activation of SREBP.'' [#kc2e6b2e]
***Kamisuki, S., Mao, Q., Abu-Eliheiga, L., Gu, Z., Kugimiya, A., Kwon, Y., Shinohara, T., Kawazoe, Y., Sato, S. Asakura, K., Choo, H., Sakai, J., Wakil, SJ., Uesugi, M. [#u8a0a574]
'''''Chem. Biol.''''' 17 (6), 616-623 (2010)&br;
https://doi.org/10.1016/j.chembiol.2010.05.015
#ref(research20.jpg,nolink,350x153)
''&br;''
''&br;''
**''A small molecule that blocks fat synthesis by inhibiting the activation of SREBP.'' [#s992b4ef]
***Kamisuki, S., Mao, Q., Abu-Eliheiga, L., Gu, Z., Kugimiya, A., Kwon, Y., Shinohara, T., Kawazoe, Y., Sato, S. Asakura, K., Choo, H., Sakai, J., Wakil, SJ., Uesugi, M. [#n83b0b39]
'''''Chem. Biol.''''' 16 (8), 882-892 (2009)&br;
https://doi.org/10.1016/j.chembiol.2009.07.007
#ref(research21.png,nolink,349x424)
''&br;''
''&br;''
**''A dumbbell-shaped small molecule that promotes cell adhesion and growth.'' [#yeae381c]
***Yamazoe, S., Shimogawa, H., Sato, S., Esko, J. D., Uesugi, M. [#xed6e7cb]
**''A dumbbell-shaped small molecule that promotes cell adhesion and growth.'' [#ge6742bb]
***Yamazoe, S., Shimogawa, H., Sato, S., Esko, J. D., Uesugi, M. [#h8352c16]
'''''Chem. Biol.''''' 16 (7), 773-782 (2009)&br;
https://doi.org/10.1016/j.chembiol.2009.06.008
#ref(research22.png,nolink,350x184)
''&br;''
''&br;''
**''Wrenchnolol derivative optimized for gene activation in cells.'' [#ldd8eea3]
***Jung, D., Shimogawa, H., Kwon, Y., Mao, Q., Sato, S., Kamisuki, S., Kigoshi, H., Uesugi, M. [#m7372468]
**''Wrenchnolol derivative optimized for gene activation in cells.'' [#w731e900]
***Jung, D., Shimogawa, H., Kwon, Y., Mao, Q., Sato, S., Kamisuki, S., Kigoshi, H., Uesugi, M. [#cfb3dd32]
'''''J. Am. Chem. Soc.''''' 131(13), 4774-4782 (2009)&br;
https://doi.org/10.1021/ja900669k
#ref(research23.gif,nolink,350x109)
''&br;''
''&br;''
**''Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.'' [#ba565fa0]
***Sato, S., Kwon, Y., Kamisuki, S., Srivastava, N., Mao, Q., Kawazoe, Y., Uesugi, M. [#uc03957f]
**''Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.'' [#b1c7adf2]
***Sato, S., Kwon, Y., Kamisuki, S., Srivastava, N., Mao, Q., Kawazoe, Y., Uesugi, M. [#hc2f23a2]
'''''J. Am. Chem. Soc.''''' 129(4), 873-880 (2007)&br;
https://doi.org/10.1021/ja0655643
#ref(research24.gif,nolink,350x161)
''&br;''
''&br;''
**''Chemical genetic identification of the IGF-linked pathway that is mediated by STAT6 and MFP2'' [#t5b122c6]
***Choi, Y., Shimogawa, H., Murakami, K., Ramdas, L., Zhang, W., Qin, J., Uesugi,M. [#ebe196ae]
**''Chemical genetic identification of histamine receptor H1 as a stimulator of insulin-induced adipogenesis.'' [#nfd9ea96]
***Kawazoe, Y., Tanaka, H., Uesugi, M. [#kc988134]
'''''Chem. Biol,''''' 13, 241-249 (2006)&br;
https://doi.org/10.1016/j.chembiol.2005.12.011
#ref(research24.jpg,nolink,350x303)
''&br;''
''&br;''
**''Chemical genetic identification of histamine receptor H1 as a stimulator of insulin-induced adipogenesis.'' [#pfbbdfa2]
***Kawazoe, Y., Tanaka, H., Uesugi, M. [#j6906713]
'''''Chem. Biol.''''' 11, 907-913 (2004)&br;
https://doi.org/10.1016/j.chembiol.2004.04.017
#ref(research25.png,nolink,350x225)
''&br;''
''&br;''
**''Small molecule transcription factor mimic.'' [#vb9d62cc]
***Kwon,Y., Arndt, H., Mao, Q., Choi, Y., Kawazoe, Y., Dervan, P. B., Uesugi, M. [#d4c9367c]
**''Small molecule transcription factor mimic.'' [#hac7c652]
***Kwon,Y., Arndt, H., Mao, Q., Choi, Y., Kawazoe, Y., Dervan, P. B., Uesugi, M. [#me114452]
'''''J. Am. Chem. Soc.''''' 126, 15940-15941 (2004)&br;
https://doi.org/10.1021/ja0445140
#ref(research26.gif,nolink,350x181)
''&br;''
''&br;''
**''A wrench-shaped synthetic molecule that modulates a transcription factor-coactivator interaction.'' [#ta2a57bf]
***Shimogawa, H., Kwon, Y., Mao, Q., Kawazoe, Y., Choi, Y., Asada, S., Kigoshi, H., & Uesugi, M. [#z86eeb0f]
**''A wrench-shaped synthetic molecule that modulates a transcription factor-coactivator interaction.'' [#rf577b72]
***Shimogawa, H., Kwon, Y., Mao, Q., Kawazoe, Y., Choi, Y., Asada, S., Kigoshi, H., & Uesugi, M. [#c36e55e3]
'''''J. Am. Chem. Soc.''''' 126, 3461-3471 (2004)&br;
https://doi.org/10.1021/ja038855+
#ref(research27.gif,nolink,350x111)
''&br;''
''&br;''
**''A gene-expression inhibitor that targets an alpha-helix-mediated protein interaction.'' [#t440c9e8]
***Asada, S., Choi, Y., & Uesugi, M. [#s08ab6ad]
**''A gene-expression inhibitor that targets an alpha-helix-mediated protein interaction.'' [#fa55b59b]
***Asada, S., Choi, Y., & Uesugi, M. [#b995b31a]
'''''J. Am. Chem. Soc.''''' 125, 4992-4993 (2003)&br;
https://doi.org/10.1021/ja0292703
#ref(research28.gif,nolink,350x145)
''&br;''
''&br;''
**''Identification of bioactive molecules by adipogenesis profiling of organic compounds.'' [#ucd49309]
***Choi, Y., Kawazoe, Y., Murakami, K., Misawa, H., & Uesugi, M [#d7fc8dff]
**''External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator.'' [#y0664646]
***Asada, S., Choi, Y., Yamada, M., Wang, S., Hung, M., Qin, J., & Uesugi, M. [#y1b7d4e8]
'''''J. Biol. Chem.''''' 278, 7320-7324 (2003)&br;
https://doi.org/10.1074/jbc.M210283200
#ref(research29.png,nolink,200x325)
''&br;''
''&br;''
**''External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator.'' [#h9833eb9]
***Asada, S., Choi, Y., Yamada, M., Wang, S., Hung, M., Qin, J., & Uesugi, M. [#o8d48eee]
'''''Proc. Natl. Acad. Sci. USA''''' 99, 12747-12752 (2002)&br;
https://doi.org/10.1073/pnas.202162199
#ref(research30.png,nolink,200x366)
''&br;''
''&br;''
**''Divergent hTAF(II)31-binding motifs hidden in activation domains.'' [#nf2de0d9]
***Choi, Y., Asada, S., & Uesugi, M. [#x19c31b3]
**''Divergent hTAF(II)31-binding motifs hidden in activation domains.'' [#z58df6a3]
***Choi, Y., Asada, S., & Uesugi, M. [#f0157fc1]
'''''J. Biol. Chem.''''' 275, 15912-15916 (2000)&br;
https://doi.org/10.1074/jbc.275.21.15912
#ref(research31.png,nolink,350x337)
''&br;''
''&br;''
**''The alpha helical FXXphiphi motif in p53: TAF interaction and discrimination by MDM2.'' [#rf2866c7]
***Uesugi, M. & Verdine, G. L. [#i08ef46f]
**''The alpha helical FXXphiphi motif in p53: TAF interaction and discrimination by MDM2.'' [#n025db74]
***Uesugi, M. & Verdine, G. L. [#n356a1cb]
'''''Proc. Natl. Acad. Sci. USA''''' 96, 14801-14806 (1999)&br;
https://doi.org/10.1073/pnas.96.26.14801
#ref(research32.png,nolink,350x324)
''&br;''
''&br;''
**''A non-natural transcriptional coactivator.'' [#b693e042]
***Nyanguile, O., Uesugi, M., Austin, D. J., & Verdine, G. L. [#if076c09]
**''A non-natural transcriptional coactivator.'' [#r5c782e2]
***Nyanguile, O., Uesugi, M., Austin, D. J., & Verdine, G. L. [#r6a8d67c]
'''''Proc. Natl. Acad. Sci. USA''''' 94, 13402-13406 (1997)&br;
https://doi.org/10.1073/pnas.94.25.13402
#ref(research33.png,nolink,350x221)
''&br;''
''&br;''
**''Induced alpha helix in the VP16 activation domain upon binding to a human TAF.'' [#y5ffb551]
***Uesugi, M., Nyanguile, O., Lu, H., Levine, A. J., & Verdine, G. L. [#d548e893]
**''Induced alpha helix in the VP16 activation domain upon binding to a human TAF.'' [#u196a48a]
***Uesugi, M., Nyanguile, O., Lu, H., Levine, A. J., & Verdine, G. L. [#r0da18e1]
'''''Science''''' 277, 1310-1313 (1997)&br;
https://doi.org/10.1126/science.277.5330.1310
#ref(research34.png,nolink,350x221)
