原著論文

Light-induced Condensation of Biofunctional Molecules around Targeted Living Cells to Accelerate Cytosolic Delivery
Ikuhiko Nakase, Moe Miyai, Kosuke Noguchi, Mamoru Tamura, Yasuyuki Yamamoto, Yushi Nishimura, Mika Omura, Kota Hayashi, Shiroh Futaki, Shiho Tokonami, Takuya Iida
Nano Lett., 22(24), 9805–9814 (2022)
DOI: 10.1021/acs.nanolett.2c02437

A Noncanonical Endocytic Pathway Is Involved in the Internalization of 3 µm Polystyrene Beads into HeLa Cells
Hisaaki Hirose, Masashi Maekawa, Hiroki Ida, Masashi Kuriyama, Yasufumi Takahashi, Shiroh Futaki
Biomaterials Sci., 10(24), 7093–7102 (2022)
DOI: 10.1039/d2bm01353c

Quantitative Analysis of Extracellular Vesicle Uptake and Fusion with Recipient Cells
Hisaaki Hirose, Yusuke Hirai, Michihito Sasaki, Hirofumi Sawa, Shiroh Futaki
Bioconjug. Chem., 33(10), 1852–1859 (2022)
DOI: 10.1021/acs.bioconjchem.2c00307

Hemopexin as a Potential Binding Partner of Arginine-rich Cell-penetrating Peptides in Serum
Kayo Nomura, Kenichi Kawano,　Yoshimasa Kawaguchi, Yuki Kawamura, Junya Michibata, Keiko Kuwata, Koji Sugiyama, Kenji Kusumoto, Shiroh Futaki
ACS Pharmacol. Trans. Sci., 5(8), 603–615 (2022)
DOI: 10.1021/acsptsci.2c00043

Split Luciferase-based Estimation of Cytosolic Cargo Concentration Delivered Intracellularly via Attenuated Cationic Amphiphilic Lytic Peptides
Syusuke Okano, Yoshimasa Kawaguchi, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Shiroh Futaki
Bioorg. Med. Chem. Lett., 72, 128875 (2022)
DOI: 10.1016/j.bmcl.2022.128875

Stearylated Macropinocytosis-inducing Peptides Facilitating the Cellular Uptake of Small Extracellular Vesicles
Yuna Nakagawa, Jan Vincent V. Arafiles, Yoshimasa Kawaguchi, Ikuhiko Nakase, Hisaaki Hirose, Shiroh Futaki
Bioconjug. Chem., 33(5), 869–880 (2022)
DOI: 10.1021/acs.bioconjchem.2c00113

Piezo1 Activation Using Yoda1 Inhibits Macropinocytosis in A431 Human Epidermoid Carcinoma Cells
Masashi Kuriyama, Hisaaki Hirose, Toshihiro Masuda, Masachika Shudou, Jan Vincent V. Arafiles, Miki Imanishi, Masashi Maekawa, Yuji Hara, Shiroh Futaki
Sci. Rep., 12, 6322 (2022)
DOI: 10.1038/s41598-022-10153-8

L17ER4: A cell-permeable attenuated cationic amphiphilic lytic peptide
Kenta Shinga, Takahiro Iwata, Kazuya Murata, Yoko Daitoku, Junya Michibata, Jan Vincent V. Arafiles, Kentarou Sakamoto, Misao Akishiba, Tomoka Takatani-Nakase, Seiya Mizuno, Fumihiro Sugiyama, Miki Imanishi, Shiroh Futaki
Bioorg. Med. Chem., 61, 116728 (2022)
DOI: 10.1016/j.bmc.2022.116728

Dodecaborate-encapsulated Extracellular Vesicles with Modification of Cell-penetrating Peptides for Enhancing Macropinocytotic Cellular Uptake and Biological Activity in Boron Neutron Capture Therapy
Shiori Hirase, Ayako Aoki, Yoshihide Hattori, Kenta Morimoto, Kosuke Noguchi, Ikuo Fujii, Tomoka Takatani-Nakase, Shiroh Futaki, Mitsunori Kirihata, Ikuhiko Nakase
Mol. Pharm., 19(4), 1135–1145 (2022)
DOI: 10.1021/acs.molpharmaceut.1c00882

Synthesis and Properties of V-Shaped Xanthene Dyes with Tunable and Predictable Absorption and Emission Wavelengths
Akari Yamagami, Kotaro Kiyotaki, Sae Wakabayashi, Naoki Egami, Kenichi Kawano, Shiroh Futaki, Ayumi Imayoshi, Kazunori Tsubaki
J. Org. Chem., 87(5), 2336–2344 (2022)
DOI: 10.1021/acs.joc.1c02139

Artificial Nanocage Formed via Self-assembly of β-Annulus Peptide for Delivering Biofunctional Pproteins into Cell Interiors
Kentarou Sakamoto, Hiroto Furukawa, Jan Vincent V. Arafiles, Miki Imanishi, Kazunori Matsuura, Shiroh Futaki
Bioconjug. Chem., 33(2), 311– 320 (2022)
DOI: 10.1021/acs.bioconjchem.1c00534

Grafting Hydrophobic Amino Acids Critical for Inhibition of Protein-Protein Interaction on a Cell-Penetrating Peptide Scaffold
Yuki Nagano, Jan Vincent V. Arafiles, Keiko Kuwata, Yoshimasa Kawaguchi, Miki Imanishi, Hisaaki Hirose, Shiroh Futaki
Mol. Pharm., 19(2), 558–567 (2022)
DOI: 10.1021/acs.molpharmaceut.1c00671

Recognition of G-quadruplex RNA by a Crucial RNA Methyltransferase Component, METTL14
Atsuhiro Yoshida, Takanori Oyoshi, Akiyo Suda, Shiroh Futaki, Miki Imanishi
Nucleic Acid Res., 50(1), 449–457 (2022)
DOI: 10.1093/nar/gkab1211

Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing Tandem Repeat Penetration-Accelerating Sequence
Akiko Okuda, Shiroh Futaki
Methods Mol. Biol., 2383, 265–273 (2022)
DOI: 10.1007/978-1-0716-1752-6_18

総説類

Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing Tandem Repeat Penetration-Accelerating Sequence
Akiko Okuda, Shiroh Futaki
Methods Mol. Biol., 2383, 265–273 (2022)
DOI: 10.1007/978-1-0716-1752-6_18

細胞膜の脂質パッキングと細胞内送達
二木史朗
オレオサイエンス, 22(3) 115–120 (2022)
DOI: 10.5650/oleoscience.22.115

原著論文

Design of the N-terminus Substituted Curvature-sensing Peptides That Exhibit Highly Sensitive Detection Ability of Bacterial Extracellular Vesicles
Kenichi Kawano, Fumiaki Yokoyama, Kouhei Kamasaka, Jun Kawamoto, Takuya Ogawa, Tatsuo Kurihara, Shiroh Futaki
Chem. Pharm. Bull., 69(11), 1075-1082 (2021)
DOI: 10.1248/cpb.c21-00516

　

Cytosolic Protein Delivery Using pH-Responsive, Charge-Reversible Lipid Nanoparticles
Yusuke Hirai, Hisaaki Hirose, Miki Imanishi, Tomohiro Asai, Shiroh Futaki
Sci. Rep., 11(1), 19896 (2021)
DOI: 10.1038/s41598-021-99180-5

Liquid droplet formation and facile cytosolic translocation of IgG in the presence of attenuated cationic amphiphilic lytic peptides
Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V. Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki
Angew. Chem. Int. Ed., 60(36), 19804–19812 (2021), (Selected as a Very Important Paper (VIP) (top 5%))
DOI: 10.1002/anie.202105527

Influence of the Dabcyl Group on the Cellular Uptake of Cationic Peptides – Short Oligoarginines as Efficient Cell-Penetrating Peptides
Ildikó Szabó, Françoise Illien, Levente E. Dókus, Mo’ath Yousef, Zsuzsa Baranyai, Szilvia Bősze, Shoko Ise, Kenichi Kawano, Sandrine Sagan, Shiroh Futaki, Ferenc Hudecz, Zoltán Bánóczi
Amino Acids, 53(7), 1033–1049 (2021)
DOI: 10.1007/s00726-021-03003-w

Membrane Anchoring of a Curvature-Inducing Peptide, EpN18, Promotes Membrane Translocation of Octaarginine
Kakeru Kuroki, Takayuki Sakai, Toshihiro Masuda, Kenichi Kawano, Shiroh Futaki
Bioorg. Med. Chem. Lett., 43, 128103 (2021)
DOI: 110.1016/j.bmcl.2021.128103

A Facile Combinatorial Approach to Construct a Ratiometric Fluorescent Sensor: Application for the Real-Time Sensing of Cellular pH Change
Eiji Nakata, Hisaaki Hirose, Khongorzul Gerelbaatar, Jan Vincent V. Arafiles, Zhengxiao Zhang, Shiroh Futaki, Takashi Morii
Chem. Sci., in press.
DOI: 10.1039/D1SC01575C

Potentiating the Membrane Interaction of an Attenuated Cationic Amphiphilic Lytic Peptide for Intracellular Protein Delivery by Anchoring with Pyrene Moiety
Kentarou Sakamoto, Junya Michibata, Yusuke Hirai, Akiko Ide, Asuka Ikitoh, Tomoka Takatani-Nakase, Shiroh Futaki
Bioconjug. Chem., 32(5), 950–957 (2021)
DOI: 10.1021/acs.bioconjchem.1c00101

Nanoscale Visualization of Morphological Alteration of Live Cell Membranes by the Interaction with Oligoarginine Cell-penetrating Peptides
Hiroki Ida, Yasufumi Takahashi, Akichika Kumatani, Hitoshi Shiku, Tomo Murayama, Hisaaki Hirose, Shiroh Futaki, Tomokazu Matsue
Anal. Chem., 93(13), 5383-5393 (2021)
DOI: 10.1021/acs.analchem.0c04097

Use of Homoarginine to Obtain Attenuated Cationic Membrane Lytic Peptides
Kentarou Sakamoto, Misao Akishiba, Takahiro Iwata, Jan Vincent V. Arafiles, Miki Imanishi, Shiroh Futaki
Bioorganic. Med. Chem. Lett., 40, 127925 (2021)
DOI: 10.1016/j.bmcl.2021.127925

Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation
Jan Vincent V. Arafiles, Hisaaki Hirose, Yusuke Hirai, Masashi Kuriyama, Maxwell Mamfe Sakyiamah, Wataru Nomura, Kazuhiro Sonomura, Miki Imanishi, Akira Otaka, Hirokazu Tamamura, Shiroh Futaki
Angew. Chem. Int. Ed., 60(21), 11928-11936 (2021)
DOI: 10.1002/anie.202016754

Development of a Microwave Resonator and Effects of Electric and Magnetic Fields on Cultured Cancer Cells
Mamiko Asano, Jun-ichi Sugiyama, Kenichi Kawano, Shiroh Futaki
Thermal Medicine, in press..

Environmental pH Stress Influences Cellular Secretion and Uptake of Extracellular Vesicles
Ikuhiko Nakase, Natsumi Ueno, Mie Matsuzawa, Kosuke Noguchi, Mami Hirano, Mika Omura, Tomoya Takenaka, Ayaka Sugiyama, Nahoko Bailey Kobayashi, Takuya Hashimoto, Tomoka Takatani-Nakase, Eiji Yuba, Ikuo Fujii, Shiroh Futaki, Tetsuhiko Yoshida
FEBS Open Bio., 11(3), 753-767 (2021)
DOI: 10.1002/2211-5463.13107

Direct Entry of Cell-Penetrating Peptide Can Be Controlled by Maneuvering the Membrane Curvature
Kazutami Sakamoto, Taku Morishita, Kenichi Aburai, Daisuke Ito, Tomonori Imura, Kenichi Sakai, Masahiko Abe, Ikuhiko Nakase, Shiroh Futaki, Hideki Sakai
Sci. Rep., 11, 31 (2021)
DOI: 10.1038/s41598-020-79518-1

総説類

Functional Peptides That Target Biomembranes: Design and Modes of Action
Shiroh Futaki
Chem. Pharm. Bull., 69(7), 601–607 (2021)

日本アカデミアでの研究例：送達ペプチドを用いた抗体の細胞内導入の試み
二木史朗
BIO Clinica, 36(6) 16–20 (2021)

マクロピノサイトーシスを活用した細胞内送達の可能性
二木史朗，広瀬久昭
生化学, 93(1), 137-140 (2021)
DOI: 10.14952/SEIKAGAKU.2021.930137

原著論文

Conversion of Cationic Amphiphilic Lytic Peptides to Cell-Penetration Peptides
Hao-Hsin Yu, Kentarou Sakamoto, Misao Akishiba, Naoki Tamemoto, Hisaaki Hirose, Ikuhiko Nakase, Miki Imanishi, Fatemeh Madani, Astrid Gräslund, Shiroh Futaki
Pept. Sci., 112(1), e24144 (2020)
DOI: 10.1002/pep2.24144

Programmable RNA Methylation and Demethylation using PUF RNA Binding Proteins
Kouki Shinoda, Akiyo Suda, Kenko Otonari, Shiroh Futaki, Miki Imanishi
Chem. Comm., 56(9), 1365–1368 (2020)
DOI: 10.1039/c9cc09298f

Stimulating Macropinocytosis for Intracellular Nucleic Acid and Protein Delivery: A Combined Strategy with Membrane-Lytic Peptides To Facilitate Endosomal Escape
Jan Vincent V. Arafiles, Hisaaki Hirose, Misao Akishiba, Shogo Tsuji, Miki Imanishi, Shiroh Futaki
Bioconjug. Chem., 31(3), 547–553 (2020) DOI: 10.1021/acs.bioconjchem.0c00064

Enhancing the Activity of Membrane Remodeling Epsin-Peptide by Trimerization
Wei-Yuan Hsu, Toshihiro Masuda, Sergii Afonin, Takayuki Sakai, Jan Vincent V. Arafiles, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Anne S. Ulrich, Shiroh Futaki
Bioorg. Med. Chem. Lett., 30(12), 127190 (2020) DOI: 10.1016/j.bmcl.2020.127190

Rational Design Principles of Attenuated Cationic Lytic Peptides for Intracellular Delivery of Biomacromolecules
Naoki Tamemoto, Misao Akishiba, Kentarou Sakamoto, Kenichi Kawano, Hiroshi Noguchi, Shiroh Futaki
Mol. Pharm., 17(6), 2175–2185 (2020) DOI: 10.1021/acs.molpharmaceut.0c00312

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature
Toshihiro Masuda, Hisaaki Hirose, Kentarou Baba, Astrid Walrant, Sandrine Sagan, Naoyuki Inagaki, Toyoshi Fujimoto, Shiroh Futaki
Bioconjug. Chem., 31(6), 1611–1615 (2020) DOI: 10.1021/acs.bioconjchem.0c00239

Improved Cytosolic Delivery of Macromolecules through Dimerization of Attenuated Lytic Peptides
Yohei Nomura, Kentarou Sakamoto, Misao Akishiba, Takahiro Iwata, Hisaaki Hirose, Shiroh Futaki
Bioorganic. Med. Chem. Lett., 30(17), 127362 (2020) DOI: 10.1016/j.bmcl.2020.127362

Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules
Kentarou Sakamoto, Misao Akishiba, Takahiro Iwata, Kazuya Murata, Seiya Mizuno, Kenichi Kawano, Miki Imanishi, Fumihiro Sugiyama, Shiroh Futaki
Angew. Chem. Int. Ed., 59(45), 19990–19998 (2020) DOI: 10.1002/anie.202005887

Pseudo-membrane Jackets: Two-dimensional Coordination Polymers Achieving Visible Phase Separation in Cell Membrane
Ryo Ohtani, Kenichi Kawano, Masanao Kinoshita, Saeko, Yanaka, Hikaru Watanabe, Kenji Hirai, Shiroh Futaki, Nobuaki Matsumori, Hiroshi Uji-I, Masaaki Ohba, Koichi Kato, Shinya Hayami
Angew. Chem. Int. Ed., 59(41), 17931–17937 (2020) DOI: 10.1002/anie.202006600

Key Process and Factors Controlling the Direct Translocation of Cell-Penetrating Peptide through Bio-membrane
Kazutami Sakamoto, Taku Morishita, Kenichi Aburai, Kenichi Sakai, Masahiko Abe, Ikuhiko Nakase, Shiroh Futaki, Hideki Sakai
Int. J. Mol. Sci., 21(15), 5466 (2020) DOI: 10.3390/ijms21155466

Development of a Simple and Rapid Method for In Situ Vesicle Detection in Cultured Media
Kenichi Kawano, Fumiaki Yokoyama, Jun Kawamoto, Takuya Ogawa, Tatsuo Kurihara, Shiroh Futaki
J. Mol. Biol., 432(22), 5876–5888 (2020) DOI: 10.1016/j.jmb.2020.09.009

Effective RNA Regulation by Combination of Multiple Programmable RNA-Binding
Misaki Sugimoto, Akiyo Suda, Shiroh Futaki, Miki Imanishi
Appl. Sci., 10(19), 6803 (2020) DOI: 10.3390/app10196803

Effect of Vesicle Particle Size on the Cytolysis of Cell-Penetrating Peptides (CPPs)
Kazutami Sakamoto, Takeshi Kitano, Haruka Kuwahara, Tomomi Tedani, Kenichi Aburai, Shiroh Futaki, Masahiko Abe, Hideki Sakai, Hiroyasu Ohtaka, Yuji Yamashita
Int. J. Mol. Sci., 21(19), 7405 (2020) DOI: 10.3390/ijms21197405

Effect of Surface Modifications on Cellular Uptake of Gold Nanorods in Human Primary Cells and Established Cell Lines
Yuxiang Xiao, Wei Xu, Yoshihiro Komohara, Yukio Fujiwara, Hisaaki Hirose, Shiroh Futaki, Takuro Niidome
ACS Omega, 5(50), 32744-32752 (2020) DOI: 10.1021/acsomega.0c05162

Identification of Synthetic Inhibitors for the DNA Binding of Intrinsically Disordered Circadian Clock Transcription Factors
Yusuke Hosoya, Wataru Nojo, Isao Kii, Takanori Suzuki, Miki Imanishi, Junko Ohkanda
Chem. Commun., 56(76), 11203-11206 (2020) DOI: 10.1039/d0cc04861e

総説類

Peptide-Assisted Intracellular Delivery of Biomacromolecules
Shiroh Futaki, Jan Vincent V. Arafiles, Hisaaki Hirose
Chem. Lett., 49(9), 1088-1094 (2020)
DOI: 10.1246/cl.200392

Design and Creation of Functional Membrane-Interacting Peptides
Shiroh Futaki
J. Syn. Org. Chem. Jpn., 78(11), 1013-1020 (2020)
DOI: 10.5059/yukigoseikyokaishi.78.1058

原著論文

Dipicolylamine/Metal Complexes that Promote Direct Cell-Membrane Penetration of Octaarginine
Yoshimasa Kawaguchi, Shoko Ise, Yusuke Azuma, Toshihide Takeuchi, Kenichi Kawano, Toan Khanh Le, Junko Ohkanda, Shiroh Futaki
Bioconjug. Chem., 30(2), 454-460 (2019)
DOI: 10.1021/acs.bioconjchem.8b00691

Inducible Membrane Permeabilization by Attenuated Lytic Peptides: A New Concept for Accessing Cell Iinteriors through Ruffled Membranes
Misao Akishiba, Shiroh Futaki
Mol. Pharm., 16(6), 2540–2548 (2019)
DOI: 10.1021/acs.molpharmaceut.9b00156

An Oligoarginine-Bearing Tandem Repeat Penetration-Accelerating Sequence Delivers Protein to the Cytosol via Caveolae-Mediated Endocytosis
Akiko Okuda, Shinya Tahara, Hisaaki Hirose, Toshihide Takeuchi, Ikuhiko Nakase, Atsushi Ono, Masanori Takehashi, Seigo Tanaka, Shiroh Futaki
Biomacromolecules, 20(5), 1849–1859 (2019) DOI: 10.1021/acs.biomac.8b01299

Cell-penetrating Mechanism of Intracellular Targeting Albumin: Contribution of Macropinocytosis Induction and Endosomal Escape
Shota Ichimizu, Hiroshi Watanabe, Hitoshi Maeda, Keisuke Hamasaki, Komei Ikegami, Victor Tuan Giam Chuang, Ryo Kinoshita, Kento Nishida, Taro Shimizu, Yu Ishima, Tatsuhiro Ishida, Takahiro Seki, Hiroshi Katsuki, Shiroh Futaki, Masaki Otagiri, Toru Maruyama
J. Control. Release., 304, 156–163 (2019) DOI: 10.1016/j.jconrel.2019.05.015

An Influenza-derived Mmembrane Tension-modulating Peptide Regulates Cell Movement and Morphology via Actin Remodeling
Toshihiro Masuda, Kentarou Baba, Takeshi Nomura, Kazuya Tsujita, Tomo Murayama, Toshiki Itoh, Tomoka Takatani-Nakase, Masahiro Sokabe, Naoyuki Inagaki, Shiroh Futaki
Commun. Biol., 2, 243 (2019) DOI: 10.1038/s42003-019-0486-3

Loosening of Lipid Packing by Cell Surface Recruitment of Amphiphilic Peptides by Coiled-coil Tethering
Takayuki Sakai, Kenichi Kawano, Masatomo Iino, Toshihide Takeuchi, Miki Imanishi, Shiroh Futaki
ChemBioChem, 20(16), 2015–2016 (2019) DOI: 10.1002/cbic.201900347

Development of a Membrane Curvature-sensing Peptide Based on a Structure–activity Correlation Study
Kenichi Kawano, Masaya Ogushi, Toshihiro Masuda, Shiroh Futaki
Chem. Pharm. Bull., 67(10), 1131–1138 (2019) DOI: 10.1248/cpb.c19-00465

Intracellular Target Delivery of Cell-penetrating Peptide-conjugated Dodecaborate for Boron Neutron Capture Therapy (BNCT)
Ikuhiko Nakase, Miku Katayama, Yoshihide Hattori, Miki Ishimura, Shunsuke Inaura, Daisuke Fujiwara, Tomoka Takatani-Nakase, Ikuo Fujii, Shiroh Futaki, Mitsunori Kirihata
Chem. Comm., 55(93), 13955–13958 (2019） DOI: 10.1039/c9cc03924d

総説類

Modified Nucleobase-Specific Gene Regulation Using Engineered Transcription Activator-Like Effectors
Shogo Tsuji, Miki Imanishi
Adv. Drug Deliv. Rev., 147, 59-65 (2019)
DOI: 10.1016/j.addr.2019.08.011

原著論文

Modular Redesign of a Cationic Lytic Peptide to Promote the Endosomal Escape of Biomacromolecules
Yusuke Azuma, Haruka Imai, Yoshimasa Kawaguchi, Ikuhiko Nakase, Hiroshi Kimura, Shiroh Futaki
Angew. Chem. Int. Ed. Eng.,,57(39), 12771–12774 (2018)
DOI: 10.1002/anie.201807534

Design and Tuning of a Cell-penetrating Albumin Derivative as a Versatile Nanovehicle for Intracellular Drug Delivery
Shota Ichimizu, Hiroshi Watanabe, Hitoshi Maeda, Keisuke Hamasaki, Yuka Nakamura, Victor Tuan Giam Chuang, Ryo Kinoshita, Kento Nishida, Ryota Tanaka, Yuki Enoki, Yu Ishima, Akihiko Kuniyasu, Yoshihiro Kobashigawa, Hiroshi Morioka, Shiroh Futaki, Masaki Otagiri, Toru Maruyama
J. Control. Release,i277(1), 23–34 (2018)
DOI: 10.1016/j.jconrel.2018.02.037

Sequence-specific 5mC Detection in Live Cells Based on the TALE-split Luciferase Complementation System
Shogo Tsuji, Kouki Shinoda, Shiroh Futaki, Miki Imanishi
Analyst,143(16), 3793–3797 (2018)
DOI: 10.1039/c8an00562a

Development of Xanthene Dyes Containing Arylacetylenes: The Role of Acetylene Linker and Substituents on the Aryl Group
Ryo Hirata, Ayame Torii, Kenichi Kawano, Shiroh Futaki, Ayumi Imayoshi, Kazunori Tsubaki
Tetrahedron,74(2), 3608–3615 (2018)
DOI: 10.1016/j.tet.2018.05.019

Importance of Net Hydrophobicity in the Cellular Uptake of All-hydrocarbon Stapled Peptides
Koki Sakagami, Toshihiro Masuda, Kenichi Kawano, Shiroh Futaki
Mol. Pharm., 15(3),1332–1340 (2018)
DOI: 10.1021/acs.molpharmaceut.7b01130

Nested PUF Proteins: Extending Target RNA Elements for Gene Regulation
Kouki Shinoda, Shogo Tsuji, Shiroh Futaki, Miki Imanishi
ChemBioChem, 15(3), 1332–1340 (2018)
DOI: 10.1002/cbic.20170045810.1039/c7cc07699a

総説類

最前線「バイオ中分子・高分子の細胞内移行・送達にかかわる新しい視点」
二木史朗
ファルマシア, 54(8), 783-787 (2018)
DOI: 10.14894/faruawpsj.54.8_783

原著論文

Detection of N6-methyladenosine Based on the Methyl-sensitivity of MazF RNA Endonuclease
Miki Imanishi, Shogo Tsuji, Akiyo Suda, Shiroh Futaki
Chem. Commun.,53(96), 12930-12933 (2017)
DOI: 10.1039/c7cc07699a

Syntheses and Properties of Second-Generation V-shaped Xanthene Dyes with Piperidino Groups
Akari Yamagami, Kenichi Kawano, Shiroh Futaki, Kouji Kuramochi, Kazunori Tsubaki
Tetrahedron,73(50), 7061–7066 (2017)
DOI: 10.1016/j.tet.2017.10.064

Loosening of Lipid Packing Promotes Oligoarginine Entry into Cells
Tomo Murayama, Toshihiro Masuda, Sergii Afonin, Kenichi Kawano, Tomoka Takatani-Nakase, Hiroki Ida, Yasufumi Takahashi, Takeshi Fukuma, Anne S. Ulrich, Shiroh Futaki
Angew. Chem. Int. Ed.,129(26), 7752–7755 (2017)
DOI: 10.1002/anie.201703578

Cytosolic Antibody Delivery by Lipid-sensitive Endosomolytic Peptide
Misao Akishiba, Toshihide Takeuchi, Yoshimasa Kawaguchi, Kentarou Sakamoto, Hao-Hsin Yu, Ikuhiko Nakase, Tomoka Takatani-Nakase, Fatemeh Madani, Astrid Gräslund, Shiroh Futaki
Nat. Chem.,9(8), 751–761 (2017)
DOI: 10.1038/NCHEM.2779

Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles for Active Macropinocytosis Induction and Efficient Intracellular Delivery
Ikuhiko Nakase, Kosuke Noguchi, Ayako Aoki, Tomoka Takatani-Nakase, Ikuo Fujii, Shiroh Futaki
Sci. Rep., 7(1), 1991 (2017)
DOI: 10.1038/s41598-017-02014-6

総説類

Cell-Surface Interactions on Arginine-Rich Cell-Penetrating Peptides Allow for Multiplex Modes of Internalization
Shiroh Futaki, Ikuhko Nakase
Acc. Chem. Res., 50(10), 2449-2456 (2017)
DOI: 10.1021/acs.accounts.7b00221

特集「生体機能性ペプチドを応用した次世代ナノメディシンの展開 アルギニンに富む膜透過ペプチドの細胞内移行
二木史朗
生化学, 89(1), 8-14 (2017)
DOI: 10.14952/SEIKAGAKU.2017.890008

原著論文

Preparation of Peptide Thioesters from Naturally Occurring Sequences Using Reaction Sequence Consisting of Regioselective S-cyanylation and Hydrazinolysis
Rin Miyajima, Yusuke Tsuda, Tsubasa Inokuma, Akira Shigenaga, Miki Imanishi, Shiroh Futaki, Akira Otaka
Biopolymers-Pept. Sci., 106(4), 531-546 (2016)

Effect of Amino Acid Substitution in the Hydrophobic Face of Amphiphilic Peptides on Membrane Curvature and Perturbation: N-Terminal Helix Derived From Adenovirus Internal Protein VI As a Model
Tomo Murayama, Silvia Pujals, Hisaaki Hirose, Ikuhiko Nakase, Shiroh Futaki
Biopolymers-Pept. Sci,106(4), 430-439 (2016)

Syndecan-4 Is a Receptor for Clathrin-mediated Endocytosis of Arginine-rich Cell-penetrating Peptides
Yoshimasa Kawaguchi, Toshihide Takeuchi, Keiko Kuwata, Junya Chiba, Yasumaru Hatanaka, Ikuhiko Nakase, Shiroh Futaki
Bioconjug. Chem., 27(4), 1119-1130 (2016)

Relating Structure and Internalization for ROMP-based Protein Mimics
Coralie M Backlund, Toshihide Takeuchi, Shiroh Futaki, Gregory Tew
Biochim. Biophys. Acta.,1858(7 Pt A), 1443-1450 (2016)

Use of Calmodulin EF-hand Peptides as Ca2+-switchable Recognition Tags
Akihiko Oku, Miki Imanishi, Daisuke Noshiro, Tomo Murayama, Toshihide Takeuchi, Ikuhiko Nakase, Shiroh Futaki
Biopolymers-Pept. Sci.,in press

Vectorization of Biomacromolecules into Cells Using Extracellular Vesicles with Enhanced Internalization Induced by Macropinocytosis
Ikuhiko Nakase, Kosuke Noguchi, Ikuo Fujii, Shiroh Futaki
Sci. Rep.,in press. 6, 34937

Cholesterol-lowering Effect of Octaarginine-appended β-Cyclodextrin in Npc1-trap-CHO Cells
Keiichi Motoyama, Rena Nishiyama, Yuki Maeda, Taishi Higashi, Yoshimasa Kawaguchi, Shiroh Futaki, Yoichi Ishitsuka, Yuki Kondo, Tetsumi Irie, Takumi Era, Hidetoshi Arima
Biol. Pharm. Bull.,39(11), 1823-1829 (2016)

Receptor Clustering and Activation by Multivalent Interaction through Recognition Peptides Presented on Exosomes
Ikuhiko Nakase, Natsumi Ueno, Miku Katayama, Kosuke Noguchi, Tomoka Takatani-Nakase, Nahoko Bailey Kobayashi, Tetsuhiko Yoshida, Ikuo Fujii, Shiroh Futaki
Chem. Commun., 53(2), 317–320 (2016)

Sequence-specific Recognition of Methylated DNA by an Engineered Transcription Activator-like Effector Protein
Shogo Tsuji, Shiroh Futaki, Miki Imanishi
Chem. Commun., 52(99), 14238–14241 (2016)

Increased Hydrophobic Block Length of PTDMs Promotes Protein Internalization
Coralie M. Backlund, Federica Sgolastra, Ronja Otter, Lisa Minter, Toshihide Takeuchi, Shiroh Futaki, Gregory N. Tew
Polym. Chem., 7(48), 7514–7521 (2016)

総説類

Current Understanding of Direct Translocation of Arginine-Rich Cell-Penetrating Peptides and its Internalization Mechanisms
Toshihide Takeuchi, Shiroh Futaki
Chem. Pharm. Bull., 64, 1431–1437 (2016)

原著論文

Combined Treatment with a pH-sensitive Fusogenic Peptide and Cationic Lipids Achieves Enhanced Cytosolic Delivery of Exosomes
Ikuhiko Nakase, Shiroh Futaki
Sci. Rep., 5, 10112 (2015)

Intercellular Chaperone Transmission via Exosomes Contributes to Maintenance of Protein Homeostasis at the Organismal Level
Toshihide Takeuchi, Mari Suzuki, Nobuhiro Fujikake, H. Akiko Popiel, Hisae Kikuchi, Shiroh Futaki, Keiji Wada, Yoshitaka Nagai
Proc. Natl. Acad. Sci. U.S.A., 112(19), E2497-E2506 (2015)

Suppressive Effect of Membrane-permeable Peptides Derived from Autophosphorylation Sites of the IGF-1 Receptor on Breast Cancer Cells
Yoshihiro Kuroda, Nahoko Kato-Kogoe, Emi Tasaki, Mayumi Yuasa-Sunagawa, Koji Yamanegi, Keiji Nakasyo, Ikuhiko Nakase, Shiroh Futaki, Yumi Tohyama, Munetaka Hirose
Eur. J. Pharm., 765, 24-33 (2015)

総説類

Cellular Uptake of Arginine-Rich Cell-Penetrating Peptides and the Contribution of Membrane-Associated Proteoglycans
Ikuhiko Nakase, Yoshimasa Kawaguchi, Motoyoshi Nomizu, Shiroh Futaki
Trends Glycosci. Glycotech., 27(155), 81-88 (2015)

Cell Penetrating Peptides for Chemical Biological Studies
Ikuhiko Nakase, Toshihide Takeuchi, Shiroh Futaki
Methods Mol. Biol., 1324, 387-396 (2015)

原著論文

Molecular Interplays Involved in the Cellular Uptake of Octaarginine on Cell Surfaces and the Importance of Syndecan-4 Cytoplasmic V Domain for the Activation of Protein Kinase Cα
Ikuhiko Nakase, Katsuhiro Osaki, Gen Tanaka, Atsushi Utani, Shiroh Futaki
Biochem. Biophys. Res. Commun. 446(4), 857-862 (2014)

Controlling Leucine-zipper Partner Recognition in Cells through Modifications of a-g Interactions
Yusuke Azuma, Tim Kükenshöner, Guangyong Ma, Jun-ichiro Yasunaga, Miki Imanishi, Gen Tanaka, Ikuhiko Nakase, Takahiro Maruno, Yuji Kobayashi, Katja M. Arndt, Masao Matsuoka, Shiroh Futaki
Chem. Commun. 50 (48), 6364-6367 (2014)

総説類

Peptide-based therapeutic approaches for treatment of the polyglutamine diseases
Toshihide Takeuchi, H. Akiko Popiel, Shiroh Futaki, Keiji Wada, Yoshitaka Nagai
Curr. Med. Chem. 21(23), 2575-2582 (2014)

細胞導入を助ける機能性ペプチド
中瀬生彦、二木史朗
化学工業,65(11), 884-890 (2014)

原著論文

Oligopeptides Derived from Autophosphorylation Sites of EGF Receptor Suppress EGF-stimulated Responses in Human Lung Carcinoma A549 cells
Yoshihiro Kurod, Nahoko Kato-Kogoe, Emi Tasaki, Eri Murata, Koyo Ueda, Mineo Abe, Kazuhide Miyamoto, Ikuhiko Nakase, Shiroh Futaki, Yumi Tohyama, Munetaka Hirose
Eur. J. Pharma. 698(1-3), 87-94 (2013)

Modeling the Endosomal Escape of Cell-Penetrating Peptides Using a Transmembrane pH Gradient
Fatemeh Madani, Rania Abdo, Staffan Lindberg, Hisaaki Hirose, Shiroh Futaki, Ülo Langel, Astrid Gräslund
Biochim. Biophys. Acta. 1828(4), 1198-1204 (2013)

Construction of a Ca2+-gated Artificial Channel by Fusing Alamethicin with a Calmodulin-derived Extramembrane Segment
Daisuke Noshiro, Kazuhiro Sonomura, Hao-Hsin Yu, Miki Imanishi, Koji Asami, Shiroh Futaki
Bioconjug. Chem. 24(2), 188-195 (2013)

Collagen-like Cell-penetrating Peptides
Chisato M. Yamazaki, Ikuhiko Nakase, Hiroyuki Endo, Saya Kishimoto, Yoshihiro Mashiyama, Ryo Masuda, Shiroh Futaki, Takaki Koide
Angew. Chem. Int. Ed. Engl. 52(21), 5497-5500

Identification of Cellular Proteins Interacting with Octaarginine (R8) Cell-penetrating Peptide by Photo-crosslinking
Yoshimasa Kawaguchi, Gen Tanaka, Ikuhiko Nakase, Miki Imanishi, Junya Chiba, Yasumaru Hatanaka, Shiroh Futaki
Bioorg. Med. Chem. Lett. 23 (13), 3738-3740 (2013)

Effects of Pyrenebutyrate on the Translocation of Arginine-rich Cell-penetrating Peptides through Artificial Membranes: Recruiting Peptides to the Membranes, Dissipating liquid-ordered Phases, and Inducing Curvature
Sayaka Katayama, Ikuhiko Nakase, Yoshiaki Yano, Tomo Murayama, Yasushi Nakata, Katsumi Matsuzaki, Shiroh Futaki
Biochim. Biophys. Acta. 1828(4), 1198-1204 (2013)

Development of a Novel Nanoparticle by Dual Modification with the Pluripotential Cell-Penetrating Peptide PepFect6 for Cellular Uptake, Endosomal Escape and Decondensation of an siRNA Core Complex
Asako Mitsueda, Yuri Shimatani, Masahiro Ito, Takashi Ohgita, Asako Yamada, Susumu Hama, Astrid Gräslund, Staffan Lindberg, Ülo Langel, Hideyoshi Harashima, Ikuhiko Nakase, Shiroh Futaki, Kentaro Kogure
Biopolymers-Peptide Science 100(6), 698-704 (2013)

Dynamic Amphiphile Libraries to Screen for the “Fragrant” Delivery of siRNA into HeLa Cells and Human Primary Fibroblasts
Charlotte Gehin, Javier Montenegro, Eun-Kyoung Bang, Ana Cajaraville, Shota Takayama, Hisaaki Hirose, Shiroh Futaki, Stefan Matile, Howard Riezman
J. Am. Chem. Soc. 135(25), 9295-9298 (2013)

Curvature Engineering: Positive Membrane Curvature Induced by Epsin N-Terminal Peptide Boosts Internalization of Octaarginine
Sílvia Pujals, Hiroki Miyamae, Sergii Afonin, Tomo Murayama, Hisaaki Hirose, Ikuhiko Nakase, Kentaro Taniuchi, Masato Umeda, Kazutami Sakamoto, Anne S. Ulrich, Shiroh Futaki
ACS Chem. Biol. 8(9), 1894-1899 (2013)

A Cyclochiral Conformational Motif Constructed Using a Robust Hydrogen Bonding Network
Kenji Mishiro, Takumi Furuta, Takahiro Sasamori, Kazuhiro Hayashi, Norihiro Tokitoh, Shiroh Futaki, Takeo Kawabata
J. Am. Chem. Soc. 135(37), 13644-13647 (2013)

Creating a TALE Protein with Unbiased 5'-T Binding
Shogo Tsuji, Shiroh Futaki, Miki Imanishi
Biochem. Biophys. Res. Commun. 441(1), 262-265 (2013)

総説類

Arginine-rich Peptides: Methods of Translocation through Biological Membranes
Shiroh Futaki, Hisaaki Hirose, Ikuhiko Nakase
Curr. Pharm. Design 19(16), 2863-2868 (2013).

Cell-penetrating Peptides (CPPs) as a Vector for the Delivery of siRNAs into Cells
Ikuhiko Nakase, Gen Tanaka, Shiroh Futaki
Mol. BioSyst. 9(5), 855-861 (2013)

Extramembrane Control of Ion Channel Peptide Assemblies, Using Alamethicin as an Example
Shiroh Futaki, Daisuke Noshiro, Tatsuto Kiwada, Koji Asami
Acc. Chem. Res. 46(12), 2924-2933 (2013)

原著論文

Cell Penetrating Peptide Induces Various Deformations of Lipid Bilayer Membrane: Inverted Micelle, Double Bilayer and Trans-membrane
Shuhei Kawamoto, Takeshi Miyakawa, Masako Takasu, Ryota Morikawa, Tatsuki Oda, Hiroaki Saito, Shiroh Futaki, Hidemi Nagao
Int. J. Quantum Chem., 112(1), 178–183 (2012)

Transient Focal Membrane Deformation Induced by Arginine-rich Peptides Leads to Their Direct Penetration into Cells
Hisaaki Hirose, Toshihide Takeuchi, Hiroko Osakada, Sílvia Pujals, Sayaka Katayama, Ikuhiko Nakase, Shouhei Kobayashi, Tokuko Haraguchi, Shiroh Futaki
Mol. Ther., 20(5), 984-993 (2012)

Accumulation of Arginine-rich Cell-penetrating Peptides in Tumors and the Potential for Anticancer Drug Delivery in vivo
Ikuhiko Nakase, Yusuke Konishi, Masashi Ueda, Hideo Saji, Shiroh Futaki
J. Control. Release, 159(2), 181-188 (2012)

The Effect of the Attachment of a Penetration Accelerating Sequence and the Influence of Hydrophobicity on Octaarginine-mediated Intracellular Delivery
Kentaro Takayama, Hisaaki Hirose, Gen Tanaka, Sílvia Pujals, Sayaka Katayama, Ikuhiko Nakase, Shiroh Futaki
Mol. Pharm., 9(5), 1222-1230 (2012)

Rational Design of DNA Sequence-specific Zinc Fingers
Hidetoshi Kono, Miki Imanishi, Shigeru Negi, Kazuya Tatsutani, Yui Sakaeda, Ayaka Hashimoto, Chie Nakayama, Shiroh Futaki, Yukio Sugiura
FEBS Lett., 586(6), 918-923 (2012)

Zn(II)-binding and DNA-binding Properties of Ligand-substituted CXHH-type Zinc Finger Proteins
Miki Imanishi, Kazushi Matsumura, Shogo Tsuji, Tomohiro Nakaya, Shigeru Negi, Shiroh Futaki, Yukio Sugiura
Biochemistry, 51, 3342-3348 (2012)

Signal Transduction of Biological Systems through an Artificial Receptor System via Dimerization of a Bivalent Leucine-Zipper Ligand
Ikuhiko Nakase, Shinya Okumura, Gen Tanaka, Katsuhiro Osaki, Miki Imanishi, Shiroh Futaki
Angew. Chem. Int. Ed. Engl., 51(30), 7464-7467 (2012)

Dipicolylamine as a Unique Structural Switching Element for Helical Peptides
Yusuke Azuma, Haruka Imai, Tomoyuki Yoshimura, Takeo Kawabata, Miki Imanishi, Shiroh Futaki
Org. Biomol. Chem., 10 (30), 6062-6068 (2012)

Bio-inspired Mechanism for the Translocation of Peptide through the Cell-membrane
Kazutami Sakamoto, Kenichi Aburai, Taku Morishita, Kenichi Sakai, Hideki Sakai, Masahiko Abe, Ikuhiko Nakase, Shiroh Futaki
Chem. Lett., in press.

CXCR4 Stimulates Macropinocytosis: Implications for Cellular Uptake of Arginine-rich Cell-penetrating Peptides and HIV
Gen Tanaka, Ikuhiko Nakase, Yasunori Fukuda, Ryo Masuda, Shinya Oishi, Kazuya Shimura, Yoshimasa Kawaguchi, Tomoka Takatani-Nakase, Ülo Langel, Astrid Gräslund, Katsuya Okawa, Masao Matsuoka, Nobutaka Fujii, Yasumaru Hatanaka, Shiroh Futaki
Chem. Biol., 19(11), 1437-1446 (2012)

Control of Leakage Activities of Alamethicin Analogs by Metals: Side Chain-dependent Adverse Gating Response to Zn2+
Daisuke Noshiro, Koji Asami, Shiroh Futaki
Bioorg. Med. Chem., 20(23), 6870-6876 (2012)

Construction of a Rhythm Transfer System That Mimics the Cellular Clock
Miki Imanishi, Kazutoshi Yamamoto, Hiroyuki Yamada, Yuka Hirose, Hitoshi Okamura, Shiroh Futaki
ACS Chem. Biol., 7(11), 1817-1821 (2012)

Induction of autophagic cell death of glioma-initiating cells by cell-penetrating d-isomer peptides consisting of Pas and the p53 C-terminus
Yutaka Ueda, Fan-Yan Wei, Taku-ichiro Hide, Hiroyuki Michiue, Kentaro Takayama, Taku Kaitsuka, Hideo Nakamura, Keishi Makino, Jun-ichi Kuratsu, Shiroh Futaki, Kazuhito Tomizawa
Biomaterials, 33(35), 9061-9069 (2012)

Transformation of an antimicrobial peptide into a plasma membrane-permeable, mitochondria-targeted peptide via the substitution of lysine with arginine
Ikuhiko Nakase, Shinya Okumura, Sayaka Katayama, Hisaaki Hirose, Sílvia Pujals, Hirofumi Yamaguchi, Satoko Arakawa, Shigeomi Shimizu, Shiroh Futaki
Chem. Commun., 48(90), 11097-11099 (2012)

総説類

Efficient Intracellular Delivery of Nucleic Acid Pharmaceuticals Using Cell-Penetrating Peptides
Ikuhiko Nakase, Hidetaka Akita, Kentaro Kogure, Astrid Gräslund, Ülo Langel, Hideyoshi Harashima, Shiroh Futaki
Acc. Chem. Res. 45(7), 1132-1139 (2012)

New functions of zinc fingers revealed by substitution of conserved residues.
Imanishi M, Sugiura Y.
Zinc Fingers: Structure, Properties and Applications (Nova Science Publishers, Inc.), 141-154 (2012)

Design of artificial DNA binding proteins toward control and elucidation of cellular functions.
Imanishi M.
Yakugaku Zasshi, in press

ゲノムを標的にした人工ジンクフィンガータンパク質の創製
今西未来
ファルマシア Vol.48, No. 3, 201-205 (2012)

膜透過性アルギニンペプチドの設計と合成
中瀬生彦、田中弦、二木史朗
遺伝子医学MOOK21 最新ペプチド合成技術とその創薬研究への応用（木曽良明編集）, 173-178 (2012)

自己分解型リンカーを用いたオリゴアルギニン修飾による薬物の消化管吸収の改善
髙山健太郎、二木史朗、林良雄、山本昌
難吸収性薬物の吸収改善と新規投与製剤の開発（山本昌監修）, 119-125 (2012)

原著論文

Inverted Micelle Formation of Cell-penetrating Peptide Studied by Coarse-grained Simulation: Importance of Attractive Force between Cell-penetrating Peptides and Lipid Head Group
Shuhei Kawamoto, Masako Takasu, Takeshi Miyakawa, Ryota Morikawa, Tatsuki Oda, Shiroh Futaki, Hidemi Nagao
J. Chem. Phys. 34(9), 095103 (2011)

Selective Isolation of N-blocked Peptide by combining AspN Digestion, Transamination, and Tosylhydrazine Glass Treatment
Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Shiroh Futaki, Osamu Nishimura
Anal. Biochem., 410(2), 214-223 (2011)

Endosomal Escape and the Knockdown Efficiency of Liposomal-siRNA by the Fusogenic Peptide shGALA
Yu Sakurai, Hiroto Hatakeyama, Yusuke Sato, Hidetaka Akita, Kentaro Takayama, Sachiko Kobayashi, Shiroh Futaki, Hideyoshi Harashima
Biomaterials, 32(24), 5733-5742 (2011)

KALA-modified Multi-layered Nanoparticles as Gene Carriers for MHC Class-I Mediated Antigen Presentation for a DNA Vaccine
Sharif M. Shaheen, Hidetaka Akita, Takashi Nakamura, Shota Takayama, Shiroh Futaki, Atsushi Yamashita, Ryo Katoono, Nobuhiko Yui, Hideyoshi Harashima
Biomaterials, 50(28), 6266-6272 (2011)

An Arginine Residue Instead of a Conserved Leucine Residue in the Recognition Helix of the Finger 3 of Zif268 Stabilizes the Domain Structure and Mediates DNA Binding
Shigeru Negi, Miki Imanishi, Maeko Sasaki, Kazuya Tatsutani, Shiroh Futaki, Yukio Sugiura
Biochemistry, 50(28), 6266-6272 (2011)

Octa-Arginine Mediated Delivery of Wild-Type Lnk Protein Inhibits TPO-Induced M-MOK Megakaryoblastic Leukemic Cell Growth by Promoting Apoptosis
Chung Yeng Looi, Miki Imanishi, Satoshi Takaki, Miki Sato, Natsuko Chiba, Yoji Sasahara, Shiroh Futaki, Shigeru Tsuchiya, Satoru Kumaki
PLosOne, 6(8), e23640 (2011)

Control of Circadian Phase by an Artificial Zinc Finger Transcription Regulator
Miki Imanishi, Atsushi Nakamura, Masao Doi, Shiroh Futaki, Hitoshi Okamura
Angew. Chem. Int. Ed. Engl., 50(40), 9396-9399 (2011)

Binding of Tat peptides on DOPC and DOPG lipid bilayer membrane studied by molecular dynamics simulations
Shuhei Kawamoto, Masako Takasu, Takeshi Miyakawa, Ryota Morikawa, Tatsuki Oda, Shiroh Futaki, Hidemi Nagao
Molecular Simulation, DOI:10.1080/08927022.2010.536546

Two-Dimensional Molecular Assembly of Bacteriochlorophyll a Derivatives Using Synthetic Poly(ethylene glycol)-Linked Light-Harvesting Model Polypeptides on a Gold Electrode Modified with Supported Lipid Bilayers
Tsuyoshi Ochiai, Morio Nagata, Kosuke Shimoyama, Tomoya Kato, Takahide Asaoka, Masaharu Kondo, Takehisa Dewa, Keiji Yamashita, Ayumi Kashiwada, Shiroh Futaki, Hideki Hashimoto, Mamoru Nango
ACS Macro Lett., 1(1), 28-32 (2011)

Acylation of Octaarginine: Implication to the Use of Intracellular Delivery Vectors
Sayaka Katayama, Hisaaki Hirose, Kentaro Takayama, Ikuhiko Nakase, Shiroh Futaki
J. Control. Release, 149 (1), 29-35 (2011)

総説類

Mechanisms of Cellular Uptake of Cell Penetrating Peptides
Fatemeh Madani, Staffan Lindberg, Ulo Langel, Shiroh Futaki, Astrid Gräslund
J. Biophys., in press.

膜透過ペプチドの生体膜透過機構と高分子の細胞内導入
二木史朗
膜 (MEMBRANE) , 36(4), 139-144 (2011)

ペプチドベクターを用いた効率的細胞導入法
二木史朗、中瀬生彦
蛍光イメージング/MRIプローブの開発（菊地和也監修）, 173-179 (2011)

原著論文

Enhanced Target-Specific Accumulation of Radiolabeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules
Rei Miyamoto, Hiromichi Akizawa, Takeshi Nishikawa, Tomoya Uehara, Yusuke Azuma, Ikuhiko Nakase, Shiroh Futaki, Hirofumi Hanaoka, Yasuhiko Iida, Keigo Endo, Yasushi Arano
Bioconjug. Chem,. 21(11), 2031-2037 (2010)

Zinc Finger-Zinc Finger Interaction between the Transcription Factors, GATA-1 and Sp1
Miki Imanishi, Chika Imamura, Chika Higashi, Wei Yan, Shigeru Negi, Shiroh Futaki, Yukio Sugiura
Biochem. Biophys. Res. Commun., 400(4), 625-630 (2010)

Transduction of Cell-Penetrating Peptides into Induced Pluripotent Stem Cells
Hiroshi Yukawa, Hirofumi Noguchi, Ikuhiko Nakase, Yoshitaka Miyamoto, Koichi Oishi, Nobuyuki Hamajima, Shiroh Futaki, Shuji Hayashi
Cell Transplant, 19(6), 901-909 (2010)

Metal-Stimulated Transcriptional Regulation by an Artificial Zinc Finger Protein
Miki Imanishi, Tomohiro Nakaya, Tatsuya Morisaki, Daisuke Noshiro, Shiroh Futaki, Yukio Sugiura
ChemBioChem, 11(12), 1653-1655 (2010)

Rev-derived Peptides Inhibit HIV-1 Replication by Antagonism of Rev and a Co-receptor, CXCR4
Kazuki Shimane, Eiichi Kodama, Ikuhiko Nakase, Shiroh Futaki, Yasuteru Sakurai, Yasuko Sakagami, Stefan G. Sarafianos, Masao Matsuoka
Int. J. Biochem. Cell Biol., 42(9), 1482-1488 (2010)

Contribution of the Flexible Loop Region to the Function of Staphylococcal Enterotoxin B
Saeko Yanaka, Motonori Kudou, Yoshikazu Tanaka, Takumi Sasaki, Sumiyo Takemoto, Atsuko Sakata, Yukio Hattori, Tomoyuki Koshi, Shiroh Futaki, Kouhei Tsumoto, Toshihiro Nakashima
Protein Eng. Des. Sel., 23(5), 415-421 (2010)

Expressed Protein Ligation for the Preparation of Fusion Proteins with Cell Penetrating Peptides for Endotoxin Removal and Intracellular Delivery
Hao-Hsin Yu, Ikuhiko Nakase, Sílvia Pujals, Hisaaki Hirose, Gen Tanaka, Sayaka Katayama, Miki Imanishi, Shiroh Futaki
Biochim. Biophys. Acta., 798(12), 2249-2257 (2010)

Nanoparticles for ex vivo siRNA Delivery to Dendritic Cells for Cancer Vaccines: Programmed Endosomal Escape and Dissociation
Hidetaka Akita, Kentaro Kogure, Rumiko Moriguchi, Yoshio Nakamura, Tomoko Higashi, Takashi Nakamura, Satoshi Serada, Minoru Fujimoto, Tetsuji Naka, Shiroh Futaki, Hideyoshi Harashima
J. Control. Release, 143(3), 311-317 (2010)

Metal-Assisted Channel Stabilization: Disposition of a Single Histidine on the N-terminus of Alamethicin Yields Channels with Extraordinarily Long Lifetimes
Daisuke Noshiro, Koji Asami, Shiroh Futaki
Biophys. J., 98(9), 1801-1808 (2010)

Cell-Penetrating d-Isomer Peptides of p53 C-Terminus: Long-term Inhibitory Effect on the Growth of Bladder Cancer
Daiji Araki, Kentaro Takayama, Miyabi Inoue, Toyohiko Watanabe, Hiromi Kumon, Shiroh Futaki, Hideki Matsui, Kazuhito Tomizawa
Urology, 75(4), 813-819 (2010)

総説類

Application of a Fusiogenic Peptide GALA for Intracellular Delivery
Ikuhiko Nakase, Kentaro Kogure, Hideyoshi Harashima, Shiroh Futaki
Methods Mol. Biol., 683, 525-533 (2010)

ペプチドをツールとして用いた細胞内への物質輸送
二木史朗 化学フロンティア 生命現象を理解する分子ツール：イメージングから生体機能解析まで（浜地格、二木史朗編）, 111-118 (2010)

Endosome-disruptive Peptides for Improving Cytosolic Delivery of Bioactive Macromolecules
Ikuhiko Nakase, Sachiko Kobayashi, Shiroh Futaki
Biopolymers (Peptide Science), 94 (6), 763-770 (2010)

膜透過性塩基性ペプチドを用いる細胞内送達技術〜その分子機構と応用〜
二木史朗, 中瀬生彦
生物物理, 50(3), 137-140 (2010)

マルチ亜鉛フィンガー型人工転写因子の創製と機能
(Artificial Transcription Factors Based on Multi-Zinc Finger Motifs)
森崎達也, 今西未来, 二木史朗, 杉浦幸雄
薬学雑誌, 130(1), 45-48 (2010)

原著論文

Development of an Intracellularly-Acting Inhibitory Peptide Selective for PKN
Kazuhiro Shiga, Kentaro Takayama, Shiroh Futaki, Jessica E. Hutti, Lewis C. Cantley, Katsuko Ueki, Yoshitaka Ono, Hideyuki Mukai
Biochem. J., 425(2), 445-453 (2009)

Elucidating Cell-Penetrating Peptide Mechanisms of Action for Membrane Interaction, Cellular Uptake, and Translocation Utilizing the Hydrophobic Counter-Anion Pyrenebutyrate
Peter Guterstam, Fatemeh Madani, Hisaaki Hirose, Toshihide Takeuchi, Shiroh Futaki, Samir EL-Andaloussi, Astrid Gräslund, Ülo Langel
Biochim. Biophys. Acta. - Biomembranes, 1788(12), 2509-2517 (2009)

Studies on the Effects of Arginine Residues Introduced to Peptide Ribonucleic Acids (PRNA) on the Complex Stability with RNA
Akihiro Nishino, Nobuya Sawa, Keiko Onodera, Seiji Sakamoto, Yasuyuki Araki, Ikuhiko Nakase, Shiroh Futaki, Yoshihisa Inoue, Takehiko Wada
Nucleic Acids Symp. Ser., (53), 55-56 (2009)

Cell Surface Accumulation of Flock House Virus-derived Peptide Leads to Efficient Internalization via Macropinocytosis
Ikuhiko Nakase, Hisaaki Hirose, Gen Tanaka, Akiko Tadokoro, Sachiko Kobayashi, Toshihide Takeuchi, Shiroh Futaki
Mol. Ther., 17(11), 1868-1876 (2009)

Positive and Negative Cooperativity of Modularly Assembled Zinc Fingers
Miki Imanishi, Atsushi Nakamura, Tatsuya Morisaki, Shiroh Futaki
Biochem. Biophys. Res. Commun., 387(3), 440-443 (2009)

Effect of Polyethyleneglycol Spacer on the Binding Properties of Nuclear Localization Signal-Modified Liposomes to Isolated Nucleus
Dai Kurihara, Hidetaka Akita, Asako Kudo, Tomoya Masuda, Shiroh Futaki, Hideyoshi Harashima
Biol. Pharm. Bull., 32(7), 1303-1306 (2009)

A pH-sensitive Fusogenic Peptide Facilitates Endosomal Escape and Greatly Enhances the Gene Silencing of siRNA-containing Nanoparticles in vitro and in vivo
Hiroto Hatakeyama, Erika Itho, Hidetaka Akita, Motoi Oishi, Yukio Nagasaki, Shiroh Futaki, Hideyoshi Harashima
J. Control. Release, 139(2), 127-132 (2009)

Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid
Yusuke Azuma, Miki Imanishi, Tomoyuki Yoshimura, Takeo Kawabata, Shiroh Futaki
Angew. Chem. Int. Ed., 48(37), 6853-6856 (2009)

Enhanced Intracellular Delivery Using Arginine-Rich Peptides by the Addition of Penetration Accelerating Sequences (Pas)
Kentaro Takayama, Ikuhiko Nakase, Hiroyuki Michiue, Toshihide Takeuchi, Kazuhito Tomizawa, Hideki Matsui, Shiroh Futaki
J. Control. Release, 138(2), 128-133 (2009)

Efficient Short Interference RNA Delivery to Tumor Cells Using a Combination of Octaarginine, GALA and Tumor-Specific, Cleavable Polyethylene Glycol System
Yu Sakurai, Hiroto Hatakeyama, Hidetaka Akita, Motoi Oishi, Yukio Nagasaki, Shiroh Futaki, Hideyoshi Harashima
Biol. Pharm. Bull., 32(5), 928-932 (2009)

Cellular Uptake, Distribution and Cytotoxicity of the Hydrophobic Cell Penetrating Peptide Sequence PFVYLI Linked to the Proapoptotic Domain Peptide PAD
Catherine L. Watkins, Paul Brennan, Christopher Fegan, Kentaro Takayama, Ikuhiko Nakase, Shiroh Futaki, Arwyn T. Jones
J. Control. Release, 140(3), 237-244 (2009)

Cytosolic Targeting of Macromolecules Using a pH-dependent Fusogenic Peptide in Combination with Cationic Liposomes
Sachiko Kobayashi, Ikuhiko Nakase, Noriko Kawabata, Hao-Hsin Yu, Sílvia Pujals, Miki Imanishi, Ernest Giralt, Shiroh Futaki
Bioconjug. Chem., 20, 953-959 (2009)
PMID: 19388672

High-resolution Multi-dimensional NMR Spectroscopy of Proteins in Human Cells
Kohsuke Inomata, Ayako Ohno, Hidehito Tochio, Shin Isogai, Takeshi Tenno, Ikuhiko Nakase, Toshihide Takeuchi, Shiroh Futaki, Yutaka Ito, Hidekazu Hiroaki, Masahiro Shirakawa
Nature, 458, 106-110 (2009)
PMID: 19262675

Low Concentration Thresholds of Plasma Membranes for Rapid Energy-Independent Translocation of a Cell Penetrating Peptide
Catherine L Watkins, Dirk Schmaljohann, Shiroh Futaki, Arwyn T Jones
Biochem. J., 420, 179-189 (2009)
PMID: 19216719

Novel System to Achieve One-Pot Modification of Cargo Molecules with Oligoarginine Vectors for Intracellular Delivery
Kentaro Takayama, Akiko Tadokoro, Sílvia Pujals, Ikuhiko Nakase, Ernest Giralt, Shiroh Futaki
Bioconjug. Chem., 20, 249-257 (2009)
PMID: 19161253

Transferrin Receptor-dependent Cytotoxicity of Artemisinin-Transferrin Conjugates on Prostate Cancer Cells and Induction of Apoptosis
Ikuhiko Nakase, Byron Gallis, Tomoka Takatani-Nakase, Steve Oh, Eric Lacoste, Narendra P Singh, David R Goodlett, Seigo Tanaka, Shiroh Futaki, Henry Lai, Tomikazu Sasaki
Cancer Lett., 274, 290-298 (2009)
PMID: 19006645

Mesostructured Silica Based Delivery System for a Drug with a Peptide as a Cell-Penetrating Vector
Chuanbo Gao, Isabel Izquierdo-Barba, Ikuhiko Nakase, Shiroh Futaki, Juanfang Ruan, Kazutami Sakamoto, Yasuhiro Sakamoto, Kazuyuki Kuroda, Osamu Terasaki, Shunai Che
Microporous and Mesoporous Materials, 122, 201-207 (2009)

総説類

膜透過ペプチドベクターの開発とメカニズム
中瀬生彦, 二木史朗
生化学, 81(11), 992-995 (2009)

Delivery of Macromolecules using Arginine-Rich Cell Penetrating Peptides: Ways to Overcome Endosomal Entrapment
Ayman El-Sayed, Shiroh Futaki, Hideyoshi Harashima
AAPS J., 11, 13-22 (2009)
PMID: 19125334

「細胞表面を取り巻く化学」
二木史朗
化学レビュー10　生体機能化学　生体機能の謎に迫る！, 64, 48-52 (2009)

原著論文

Oligoarginine-Based Prodrugs with Self-Cleavable Spacers for Caco-2 Cell Permeation
Kentaro Takayama, Yuka Suehisa, Takuya Fujita, Jeffrey-Tri  Nguyen, Shiroh Futaki, Akira Yamamoto, Yoshiaki Kiso, Yoshio Hayashi
Chem. Pharm. Bull., 56(11), 1515-1520 (2008)
PMID: 18981599

Effects of bulkiness and hydrophobicity of an aliphatic amino acid in the recognition helix of the GAGA zinc finger on the stability of the hydrophobic core and DNA binding affinity
Muthu Dhanasekaran, Shigeru Negi, Miki Imanishi, Michiko Suzuki, Yukio Sugiura
Biochemistry, 47(45), 11717-11724 (2008)
PMID: 18855425

Effects of Arginine Residue Introduction upon Interaction and Complexation Behavior of Peptide Ribonucleic Acids (PRNAs) with RNA: Synthesis and Properties of alpha-Containing arginine
Takehiko Wada, Nobuya Sawa, Hirofumi Sato, Shiroh Futaki, Yoshihisa Inoue.
Nucleic Acids Symp. Ser., (52), 673-674 (2008)
PMID: 18776559

Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by Artificial Multi-Zinc Finger Protein
Tatsuya Morisaki, Miki Imanishi, Shiroh Futaki, Yukio Sugiura
Biochemistry, 47(38), 10171-10177 (2008)
PMID: 18754679

Octaarginine- and Octalysine-Modified Nanoparticles Have Different Modes of Endosomal Escape
Ayman El-Sayed, Ikramy A. Khalil, Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima
J. Biol. Chem., 283(34), 23450-23461 (2008)
PMID: 18550548

An Artificial Virus-like Nano Carrier System: Enhanced Endosomal escape of Nanoparticles via Synergistic Action of pH-sensitive Fusogenic Peptide Derivatives
Kentaro Sasaki, Kentaro Kogure, Shinji Chaki, Yoshio Nakamura, Rumiko Moriguchi, Hirofumi Hamada, Radostin Danev, Kuniaki Nagayama, Shiroh Futaki, Hideyoshi Harashima
Anal. Bioanal. Chem., 391(8), 2717-2727 (2008)
PMID: 18351325

Cellular Internalization and Distribution of Arginine-Rich Peptides as a Function of Extracellular Peptide Concentration, Serum, and Plasma Membrane Associated Proteoglycans
Michie Kosuge, Toshihide Takeuchi, Ikuhiko Nakase, Arwyn Tomos Jones, Shiroh Futaki
Bioconjug. Chem. 19(3), 656-664 (2008)
PMID: 18269225

総説類

Octaarginine-modified Liposomes: Enhanced Cellular Uptake and Controlled Intracellular Trafficking
Ikramy A. Khalil, Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima
Int. J. Pharm., 354(1-2), 39-48 (2008)
PMID: 18242018

New Redesigned Zinc-Finger Proteins: Design Strategy and Its Application
Shigeru Negi, Miki Imanishi, Makoto Matsumoto, Yukio Sugiura
Chemistry, 14(11), 3236-3249 (2008)
PMID: 18236477

Membrane Permeable Peptide Vectors: Chemistry and Functional Design for the Therapeutic Applications
Shiroh Futaki
Adv. Drug Deliv. Rev., 60(4-5), 447 (2008)
PMID: 18077052

Methodological and Cellular Aspects that Govern the Internalization Mechanisms of Arginine-rich Cell-penetrating Peptides
Ikuhiko Nakase, Toshihide Takeuchi, Gen Tanaka, Shiroh Futaki
Adv. Drug Deliv. Rev., 60(4-5), 598-607 (2008)
PMID: 18045727

Anticancer properties of artemisinin derivatives and their targeted delivery by transferrin conjugation
Ikuhiko Nakase, Henry Lai, Narendra P. Singh, Tomikazu Sasaki
Int. J. Pharm., 354(1-2), 28-33 (2008)
PMID: 17942255

セミナー：膜透過ペプチドを用いる細胞導入技術
二木史朗
ファルマシア, 44(4), 321-325 (2008)

原著論文

Development of Oligoarginine-drug Conjugates Linked to New Peptidic Self-cleavable Spacers toward Effective Intestinal Absorption
Yoshio Hayashi, Kentaro Takayama, Yuka Suehisa, Takuya Fujita, Jeffrey-Tri Nguyen, Shiroh Futaki, Akira Yamamoto, Yoshiaki Kiso
Bioorg. Med. Chem. Lett., 17(18), 5129-5132 (2007)
PMID: 17662604

Guanidine-Containing Molecular Transporters: Sorbitol-Based Transporters Show High Intracellular Selectivity toward Mitochondria
Kaustabh K. Maiti, Woo Sirl Lee, Toshihide Takeuchi, Catherine Watkins, Marjan Fretz, Dong-Chan Kim, Shiroh Futaki, Arwyn Jones, Kyong-Tai Kim, Sung-Kee Chung
Angew. Chem. Int. Ed. Engl., 46(31), 5880-5884 (2007)
PMID: 17607670

Alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence
Wei Yan, Miki Imanishi, Shiroh Futaki, Yukio Sugiura
Biochemistry, 46(29), 8517-8524 (2007)
PMID: 17602503

Octaarginine-modified Multifunctional Envelope-type Nano Device for siRNA
Yoshio Nakamura, Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima
J. Control. Release, 119(3), 360-367 (2007)
PMID: 17478000

New Packaging Method of Mycobacterial Cell Wall using Octaarginine-modified Liposomes: Enhanced Uptake by and Immunostimulatory Activity of Dendritic Cells
Atthachai Homhuan, Kentaro Kogure, Hideyuki Akaza, Shiroh Futaki, Takashi Naka, Yukiko Fujita, Ikuya Yano, Hideyoshi Harashima
J. Control. Release, 120(1-2), 60-69 (2007)
PMID: 17467840

NMR Investigation of the Electrostatic Effect in Binding of a Neuropeptide, Achatin-I, to Phosphatidylcholine Bilayers
Tomohiro Kimura, Keiko Ninomiya, Shiroh Futaki
J. Phys. Chem. B. 111(14), 3831-3838 (2007)
PMID: 17388516

Octaarginine-modified Multifunctional Envelope-type Nanoparticles for Gene Delivery
I. A. Khalil, K. Kogure, S. Futaki, S. Hama, H. Akita, M. Ueno, H. Kishida, M. Kudoh, Y. Mishina, K. Kataoka, M. Yamada, H. Harashima
Gene Ther., 14(8), 682-689 (2007)
PMID: 17268535

The Use of Acid Wash in Determining Cellular Uptake of Fab/Cell-Permeating Peptide Conjugates
Shouju Kameyama, Mayo Horie, Takeo Kikuchi, Takao Omura, Akiko Tadokoro, Toshihide Takeuchi, Ikuhiko Nakase, Yukio Sugiura, Shiroh Futaki
Biopolymers (Peptide Science), 88(2), 98-106 (2007)
PMID: 17252560

Activation of JNK pathway during islet transplantation and prevention of islet graft loss by intraportal injection of JNK inhibitor
H. Noguchi, Y. Nakai, M. Ueda, Y. Masui, S. Futaki, N. Kobayashi, S. Hayashi, S. Matsumoto
Diabetologia, 50(3), 612-619 (2007)
PMID: 17225125

Temperature, Concentration and Cholesterol Dependent Translocation of L- and D-octaarginine across the Plasma and Nuclear membrane of CD34+ Leukaemia Cells
Marjan M. Fretz, Neal A. Penning, Saly Al-Taei, Shiroh Futaki, Toshihide Takeuchi, Ikuhiko Nakase, Gert Storm, Arwyn T. Jones
Biochemical J., 403(2), 335-342 (2007)
PMID: 17217340

Interaction of Arginine-Rich Peptides with Membrane-Associated Proteoglycans Is Crucial for Induction of Actin Organization and Macropinocytosis
Ikuhiko Nakase, Akiko Tadokoro, Noriko Kawabata, Toshihide Takeuchi, Hironori Katoh, Kiyo Hiramoto, Manabu Negishi, Motoyoshi Nomizu, Yukio Sugiura, Shiroh Futaki
Biochemistry, 46(2), 492-501 (2007)
PMID: 17209559

総説類

Arginine-rich peptides and their internalization mechanisms
Shiroh Futaki, Ikuhiko Nakase, Akiko Tadokoro, Toshihide Takeuchi Arwyn Tomos Jones
Biochem. Soc. Trans. 35(Pt 4), 784-787 (2007)
PMID: 17635148

Ligand-Induced Extramembrane Conformation Switch Controlling Alamethicin Assembly and the Channel Current
Shiroh Futaki, Koji Asami
Chem. Biodiv. 4(6), 1313-1322 (2007)
PMID: 17589883

原著論文

Oligomers of Beta-Amino Acid Bearing Non-planar Amides Form Ordered Structures
Yuko Otani, Shiroh Futaki, Tatsuto Kiwada, Yukio Sugiura, Atsuya Muranaka, Nagao Kobayashi, Masanobu Uchiyama, Kentaro Yamaguchi, Tomohiko Ohwada
Tetrahedron, 62(50), 11635-11644 (2006)

Direct and Rapid Cytosolic Delivery Using Cell-Penetrating Peptides Mediated by Pyrenebutyrate
Toshihide Takeuchi, Michie Kosuge, Akiko Tadokoro, Yukio Sugiura, Mayumi Nishi, Mitsuhiro Kawata, Naomi Sakai, Stefan Matile, Shiroh Futaki
ACS Chem. Biol., 1(5), 299-303 (2006)
PMID: 17163758

Autoinhibition Regulates the Motility of the C. Elegans Intraflagellar Transport Motor OSM-3
Miki Imanishi, Nicholas F. Endres, Arne Gennerich, Ronald D. Vale
J. Cell Biol., 174(7), 931-937 (2006)
PMID: 17000874

Effects of Na+/H+ Exchanger Inhibitors on Subcellular Localisation of Endocytic Organelles and Intracellular Dynamics of Protein Transduction Domains HIV-TAT Peptide and Octaarginine
Marjan Fretz, Jing Jin, Robin Conibere, Neal A. Penning, Saly Al-Taei, Gert Storm, Shiroh Futaki, Toshihide Takeuchi, Ikuhiko Nakase, Arwyn T. Jones
J. Control. Release, 116(2), 247-254 (2006)
PMID: 16971016

Cellular Uptake and Subsequent Intracellular Trafficking of R8-liposomes Introduced at Low Temperature
Akitada Iwasa, Hidetaka Akita, Ikramy Khalil, Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima
Biochim. Biophys. Acta - Biomembranes, 1758(6), 713-720 (2006)
PMID: 16780792

Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells
Takashi Nakamura, Rumiko Moriguchi, Kentaro Kogure, Arisa Minoura, Tomoya Masuda, Hidetaka Akita, Kazunori Kato, Hirofumi Hamada, Masaharu Ueno, Shiroh Futaki, Hideyoshi Harashima
Biol. Pharm. Bull., 29(6), 1290-1293 (2006)
PMID: 16755037

Effects of Cell-Permeating Peptide Binding on the Distribution of 125I-Labeled Fab Fragment in Rats
Shoju Kameyama, Mayo Horie, Takeo Kikuchi, Takao Omura, Toshihide Takeuchi, Ikuhiko Nakase, Yukio Sugiura, Shiroh Futaki
Bioconjug. Chem., 17(3), 597-602 (2006)
PMID: 16704196

Transmission of Extramembrane Conformational Change into Current; Construction of Metal-gated Ion Channel
Tatsuto Kiwada, Kazuhiro Sonomura, Yukio Sugiura, Koji Asami, Shiroh Futaki
J. Am. Chem. Soc., 128(18), 6010-6011 (2006)
PMID: 16669650

Significant and Prolonged Antisense Effect of a Multifunctional Envelope-type Nano Device Encapsulating Antisense Oligodeoxynucleotide
Yoshio Nakamura, Kentaro Kogure, Yuma Yamada, Shiroh Futaki, Hideyoshi Harashima,
J. Pharm. Pharmacol., 58(4), 431-437 (2006)
PMID: 16597360

Distribution of Immunoglobulin Fab Fragment Conjugated with HIV-1 REV Peptide Following Intravenous Administration in Rats
Shouju Kameyama, Ritsuko Okada, Takeo Kikuchi, Takao Omura, Ikuhiko Nakase, Toshihide Takeuchi, Yukio Sugiura, Shiroh Futaki
Mol. Pharm. 3(2), 174-180 (2006)
PMID: 16579646

Design, Synthesis, and Membrane-Translocation Studies of Inositol-Based Transporters
Kaustabh K. Maiti, Ock-Youm Jeon, Woo Sirl Lee, Dong-Chan Kim, Kyong-Tai Kim, Toshihide Takeuchi, Shiroh Futaki, Sung-Kee Chung
Angew. Chem. Int. Ed. Engl., 45(18), 2907-2912 (2006)
PMID: 16555349

Intracellular Traffic and Fate of Protein Transduction Domains HIV-1 TAT Peptide and Octaarginine. Implications for their Utilization as Drug Delivery Vectors
Saly Al Taei, Neal A. Penning, Jeremy C. Simpson, Shiroh Futaki, Toshihide Takeuchi, Ikuhiko Nakase, Arwyn T. Jones
Bioconjug. Chem., 17(1), 90-100 (2006)
PMID: 16417256

High Density of Octaarginine Stimulates Macropinocytosis Leading to Efficient Intracellular Trafficking for Gene Expression
Ikramy A. Khalil, Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima
J. Biol. Chem., 281(6), 3544-3551 (2006)
PMID: 16326716

Evaluation of the Nuclear Delivery and Intra-nuclear Transcription of Plasmid DNA Condensed with micro (mu) and NLS-micro by Cytoplasmic and Nuclear Microinjection: A Comparative Study with Poly-L-lysine
Hidetaka Akita, Mitsuhide Tanimoto, Tomoya Masuda, Kentaro Kogure, Susumu Hama, Keiko Ninomiya, Shiroh Futaki, Hideyoshi Harashima
J. Gene Med., 8(2), 198-206 (2006)
PMID: 16285003

総説類

分子集合体の細胞内デリバリー
二木史朗
臨床検査, 50(12), 1439-1546 (2006) （臨床検査増刊号,ナノテクノロジーとバイオセンサ）

Oligoarginine Vectors for Intracellular Delivery: Design and Cellular-uptake Mechanisms
Shiroh Futaki
Biopolymers (Peptide Science), 84(3), 241-249 (2006)
PMID: 16333858

Anion Hopping of (and on) Functional Oligoarginines: From Chloroform to Cells
Naomi Sakai, Shiroh Futaki, Stefan Matile
Soft Matter, 2(8), 636-641 (2006)

トピックス：ペプチドベクターを用いた細胞内移送
二木史朗
酵素工学ニュース第55号, 平成18年4月号, 8-11

ペプチドによる細胞内移送制御
二木史朗
高分子, 55(5), 345 (2006)

遺伝子を標的とした人工亜鉛フィンガータンパク質の創製
今西未来
化学工業, 57(9), 667-671 (2006)

原著論文

PDX-1 Protein Is Internalized by Lipid Raft-dependent Macropinocytosis
Hirofumi Noguchi, Shinichi Matsumoto, Teru Okitsu, Yasuhiro Iwanaga, Yukihide Yonekawa, Hideo Nagata, Masayuki Matsushita, Fan-Yan Wei, Hideki Matsui, Kohtaro Minami, Susumu Seino, Yumi Masui, Shiroh Futaki, Koichi Tanaka
Cell Transplantation, 14(9), 637-645 (2005)
PMID: 16405074

Real-time In-cell ¹⁹F NMR Study on Uptake of Fluorescent and Nonfluorescent ¹⁹F-Octaarginines into Human Jurkat Cells
Emiko Okamura, Keiko Ninomiya, Shiroh Futaki, Yasuharu Nagai, Tomohiro Kimura, Chihiro Wakai, Nobuyuki Matubayasi, Yukio Sugiura, Masaru Nakahara
Chem. Lett., 34(7), 1064-1065 (2005)

Pharmacokinetic Analysis of the Tissue Distribution of Octaarginine Modified Liposomes in Mice
Diky Mudhakir, Hidetaka Akita, Ikramy A. Khalil, Shiroh Futaki, Hideyoshi Harashima
Drug Metab. Pharmacokinet., 20(4), 275-281 (2005)
PMID: 16141607

Mitochondrial Delivery of Mastoparan with Transferrin Liposomes Equipped with a pH-sensitive Fusogenic Peptide for Selective Cancer Therapy
Yuma Yamada, Yasuo Shinohara, Tomoyuki Kakudo, Shinji Chaki, Shiroh Futaki, Hiroyuki Kamiya, Hideyoshi Harashima
Int. J. Pharm., 303(1-2), 1-7 (2005)
PMID: 16139452

Unique Features of a pH-sensitive Fusogenic Peptide that Improves the Transfection Efficiency of Cationic Liposomes
Shiroh Futaki, Yumi Masui, Ikuhiko Nakase, Yukio Sugiura, Takashi Nakamura, Kentaro Kogure, and Hideyoshi Harashima
J. Gene Med., 7(11), 1450-1458 (2005)
PMID: 16025556

A Multifunctional Envelope-type Nano Device for Novel Gene Delivery of siRNA Plasmids.
Rumiko Moriguchi, Kentaro Kogure, Hidetaka Akita, Shiroh Futaki, Makoto Miyagishi, Kazunari Taira, Hideyoshi Harashima
Int. J. Pharm., 301(1-2), 277-85 (2005)
PMID: 16019173

Cell Permeable Peptide of JNK Inhibitor Prevents Islet Apoptosis Immediately after Isolation and Improves Islet Graft Function
Hirofumi Noguchi, Yusuke Nakai, Shinichi Matsumoto, Miho Kawaguchi, Michiko Ueda, Teru Okitsu, Yasuhiro Iwanaga, Yukihide Yonekawa, Hideo Nagata, Kohtaro Minami, Susumu Seino, Yumi Masui, Shiroh Futaki, Koichi Tanaka
Am. J. Transplant., 5(8), 1848-1855 (2005)
PMID: 15996231

An Artificial Six-Zinc Finger Peptide With Polyarginine Linker: Selective Binding to the Discontinuous DNA Sequences
Miki Imanishi, Yan Wei, Tatsuya Morisaki, Yukio Sugiura
Biochem Biophys Res Commun., 333(1), 167-73 (2005)
PMID: 15939400

Arginine Magic With New Counterions Up the Sleeve
Masamichi Nishihara, Florent Perret, Toshihide Takeuchi, Shiroh Futaki, Adina N. Lazar, Anthony W. Coleman, Naomi Sakai, Stefan Matile
Org. Biomol. Chem., 3(9), 1659-1669 (2005)
PMID: 15858647

Effects of Linking 15-Zinc Finger Domains on DNA Binding Specificity and Multiple DNA Binding Modes
Tsuyoshi Hirata, Wataru Nomura, Miki Imanishi, Yukio Sugiura
Bioorg Med Chem Lett., 15(9), 2197-2201 (2005)
PMID: 15837293

Site-Specific DNA Cleavage by Artificial Zinc Finger-Type Nuclease with Cerium-Binding Peptide
Takako Nakatsukasa, Yasuhisa Shiraishi, Shigeru Negi, Miki Imanishi, Shiroh Futaki, Yukio Sugiura
Biochem. Biophys. Res. Commun., 330(1), 247-252 (2005)
PMID: 15781257

Swapping of the Beta-Hairpin Region between Sp1 and GLI Zinc Fingers: Significant Role of the Beta-Hairpin Region in DNA Binding Properties of C2H2-Type Zinc Finger Peptides
Yasuhisa Shiraishi, Miki Imanishi, Tatsuya Morisaki, Yukio Sugiura
Biochemistry, 44(7), 2523-2528 (2005)
PMID: 15709764

Anionic Fullerenes, Calixarenes, Coronenes, and Pyrenesas Activators of Oligo/Polyarginines in Model Membranes and Live Cells
Florent Perret, Masamichi Nishihara, Toshihide Takeuchi, Shiroh Futaki, Adina N. Lazar, Anthony W. Coleman, Naomi Sakai, Stefan Matile
J. Am. Chem. Soc., 127(4), 1114-1115 (2005)
PMID: 15669846

総説類

膜透過ペプチドを用いた蛋白質細胞内移送
二木史朗
化学と生物, 43(10), 649-653 (2005)

塩基性ペプチドとのハイブリッド化によるタンパク質・薬物の効率的細胞内導入
二木史朗
Bio Industry, 22(3), 34-39 (2005)

Membrane-permeable Arginine-rich Peptides and the Translocation Mechanisms
Shiroh Futaki
Adv. Drug Deliv. Rev., 57(4), 547-558 (2005)
PMID: 15722163

原著論文

NMR Structure of Transcription Factor Sp1 DNA Binding Domain
Shinichiro Oka, Yasuhisa Shiraishi, Takuya Yoshida, Tadayasu Ohkubo, Yukio Sugiura, Yuji Kobayashi
Biochemistry, 43(51), 16027-16035 (2004)
PMID: 15609997

Control of Peptide Structure and Recognition by Fe(III)-induced Helix Destabilization
Shiroh Futaki, Tatsuto Kiwada, Yukio Sugiura
J. Am. Chem. Soc., 126(48), 15762-15769 (2004)
PMID: 15571399

Cellular Uptake of Arginine-Rich Peptides: Roles for Macropinocytosis and Actin Rearrangement
Ikuhiko Nakase, Miki Niwa, Toshihide Takeuchi, Kazuhiro Sonomura, Noriko Kawabata, Yukihiro Koike, Masanori Takehashi, Seigo Tanaka, Kunihiro Ueda, Jeremy C. Simpson, Arwyn T. Jones, Yukio Sugiura, Shiroh Futaki
Mol. Ther., 10(6), 1011-1022 (2004)
PMID: 15564133

Direct Observation of Anion-Mediated Translocation of Fluorescent Oligoarginine Carriers Into and Across Bulk Liquid and Anionic Bilayer Membranes
Naomi Sakai, Toshihide Takeuchi, Shiroh Futaki, Stefan Matile
ChemBioChem, 6(1), 114-122 (2005)
(Published on-line: November 17, 2004)
PMID: 15549725

Creation and Characteristics of Unnatural CysHis(3)-Type Zinc Finger Protein
Shigeru Negi, Masako Itazu, Miki Imanishi, Akiko Nomura, Yukio Sugiura
Biochem Biophys Res Commun., 325(2), 421-425 (2004)
PMID: 15530409

RNase S Complex Bearing Arginine-rich Peptide and Anti-HIV Activity
Shiroh Futaki, Ikuhiko Nakase, Tomoki Suzuki, Daisuke Nameki, Ei-ichi Kodama, Masao Matsuoka, Yukio Sugiura
J. Mol. Recogn., 18(2), 169-174 (2004)
PMID: 15476294

Development of a Non-viral Multifunctional Envelop-Type Nano Device by a Novel Lipid Film Hydration Method
Kentaro Kogure, Rumiko Moriguchi, Kentaro Sasaki, Masaharu Ueno, Shiroh Futaki, Hideyoshi Harashima
J. Control. Release, 98(2), 317-323 (2004)
PMID: 15262422

Exchange of Histidine Spacing Between Sp1 and GLI Zinc Fingers: Distinct Effect of Histidine Spacing-Linker Region on DNA Binding
Yasuhisa Shiraishi, Miki Imanishi, Yukio Sugiura
Biochemistry, 43(20), 6352-6359 (2004)
PMID: 15147220

Arginine Carrier Peptide Bearing Ni(II) Chelator to Promote Cellular Uptake of Histidine-Tagged Proteins
Shiroh Futaki, Miki Niwa, Ikuhiko Nakase, Akiko Tadokoro, Youjun Zhang, Makoto Nagaoka, Noaya Wakako, Yukio Sugiura
Bioconjug. Chem., 15(3), 475-481 (2004)
PMID: 15149174

Transferrin-modified Liposomes Equipped with a pH-sensitive Fusogenic Peptide: an Artificial Viral-like Delivery System
Tomoyuki Kakudo, Shinji Chaki, Shiroh Futaki, Ikuhiko Nakase, Kenichi Akaji, Toru Kawakami, Kazuo Maruyama, Hiroyuki Kamiya, Hideyoshi Harashima
Biochemistry, 43(19), 5618-5628 (2004)
PMID: 15134436

Total Synthesis of Artificial Zinc Finger Proteins: Problems and Perspectives
Shiroh Futaki, Tatsuto Kiwada, Yasuhisa Shiraishi, Yukio Sugiura
Biopolymers (Peptide Science), 76(2), 98-109 (2004)
PMID: 15054890

Gramicidin-based Channel Systems for the Detection of Protein-ligand Interaction
Shiroh Futaki, Youjun Zhang, Tatsuto Kiwada, Ikuhiko Nakase, Takeshi Yagami, Shigetoshi Oiki, Yukio Sugiura
Bioorg. Med. Chem., 12(6), 1343-1350 (2004)
PMID: 15018906

Quantitative Three-dimensional Analysis of the Intracellular Trafficking of Plasmid DNA Transfected by Non-viral Gene Delivery System using Confocal Laser Scanning Microscopy
Hidetaka Akita, Rie Ito, Ikramy A. Khalil, Shiroh Futaki, Hideyoshi Harashima
Mol. Ther., 9(3), 443-451 (2004)
PMID: 15006612

Total Synthesis of Large CCK Isomers Using a Thioester Segment Condensation Approach
Kouki Kitagawa, Hiroshi Adachi, Yuki Sekigawa, Takeshi Yagami, Shiroh Futaki, Yuan Jun Gu, Kazutomo Inoue
Tetrahedron, 60(4), 907-918 (2004)

Mechanism of Improved Gene Transfer by the N-Terminal Stearylation of Octaarginine: Enhanced Cellular association by Hydrophobic Core Formation
Ikramy A. Khalil, Shiroh Futaki, Miki Niwa, Yoshinobu Baba, Noridata Kaji, Hiroyuki Kamiya, Hideyoshi Harashima
Gene Ther., 11(7), 636-644 (2004)
PMID: 14973542

Alpha, Alpha-Disubstituted Glycines Bearing a Large Hydrocarbon Ring. Peptide Self-Assembly through Novel Hydrophobic Recognition
Tomohiko Ohwada, Daisuke Kojima, Tatsuto Kiwada, Shiroh Futaki, Yukio Sugiura, Kentaro Yamaguchi, Yoshinori Nishi, Yuji Kobayashi
Chemistry- A Eur J., 10(3) 617-626 (2004)
PMID: 14767925

総説類

ペプチドによるドラッグデリバリー
二木史朗
細胞, 36(10), 428-431 (2004)

アルギニンペプチドによる細胞内デリバリー
二木史朗
薬剤学, 64(3), 164-167 (2004)

原著論文

pH-sensitive Liposomes in Nuclear Targeting of Macromolecules
Rieko Tachibana, Shiroh Futaki, Hideysoshi Harashima, Hiroshi Kiwada
Methods Enzymol., 372, 349-361(2003)
PMID: 14610823

Synthetic Copoly(Lys/Phe) and Poly(Lys) Translocate through Lipid Bilayer Membranes
Akira Shibata, Syuichi Murata, Satoru Ueno, Shaoqian Liu, Shiroh Futaki, Yoshinobu Baba
Biochim. Biophys, Acta - Biomembranes, 1616(2), 147-155 (2003)
PMID: 14561472

DNA Cleavage Characteristics of Non-protein Enediyne Antibiotic N1999A2
Naoko Miyagawa, Daisuke Sasaki, Mieko Matsuoka, Miki Imanishi, Toshihiko Ando, Yukio Sugiura
Biochem Biophys Res Commun., 306(1), 87-92 (2003)
PMID: 12788070

Can Nuclear Localization Signals Enhance Nuclear Localization of Plasmid DNA?
Takeshi Nagasaki, Teruhiko Myohoji, Taro Tachibana, Shiroh Futaki, Seizo Tamagaki
Bioconjug. Chem., 14(2), 282-286 (2003)
PMID: 12643737

Influence of TFIIIA-type Linker at the N- or C-terminal of Nine-zinc Finger Protein on DNA-Binding Site
Wataru Nomura, Makoto Nagaoka, Yasuhisa Shiraishi, Yukio Sugiura
Biochem Biophys Res Commun., 300(1), 87-92 (2003)
PMID: 12480525

総説類

Membrane-permeability Commonly Shared among Arginine-rich Peptides
Shiroh Futaki, Susumu Goto, Yukio Sugiura
J. Mol. Recog. 16(5), 260 - 264 (2003)
PMID: 14523938

細胞内への物質導入
二木史朗
遺伝子医学別冊「ドラッグデリバリーシステムDDS技術の新たな展開とその活用法」（田畑泰彦編集）, 18-22 (2003)

ペプチドによるドラッグデリバリー
二木史朗
現代医療, 75(7) 233-238 (2003)

Structural Variety of Membrane Permeable Peptides
Shiroh Futaki, Susumu Goto, Tmoki Suzuki, Ikuhiko Nakase, Yukio Sugiura
Curr. Prot. Pept. Sci., 4(2) 87-96 (2003)
PMID: 12678848

膜透過ペプチドを用いた生体高分子の細胞内輸送
二木史朗
膜, 28(2) 55-60 (2003)

原著論文

Generation of Reactive Oxygen Species and Activation of NF-kappaB by non-Abeta Component of Alzheimer's Disease Amyloid
Seigo Tanaka, Masanori Takehashi, Naomi Matoh, Shinya Iida, Tomoki Suzuki, Shiroh Futaki, Hirofumi Hamada, Eliezer Masliah, Yukio Sugiura, Kunihiro Ueda
J. Neurochem., 82(2), 305-315 (2002)
PMID: 12124431

Interconversion between Serine and Aspartic Acid in the Alpha Helix of the N-terminal Zinc Finger of Sp1: Implication for General Recognition Code and for Design of Novel Zinc Finger Peptide Recognizing Complementary Strand
Makoto Nagaoka, Yasuhisa Shiraishi, Yumiko Uno, Wataru Nomura, Yukio Sugiura
Biochemistry, 41(28), 8819-8825 (2002)
PMID: 12102624

Translocation of Branched-chain Arginine Peptides through Cell Membranes: Flexibility in the Spatial Disposition of Positive Charges in Membrane-permeable Peptides
Shiroh Futaki, Ikuhiko Nakase, Tomoki Suzuki, Youjun Zhang, Yukio Sugiura
Biochemistry, 41(25), 7925-7930 (2002)
PMID: 12069581

Detection of Protein-ligand Interaction on the Membranes Using C-terminus Biotin-tagged Alamethicin
Zhang Youjun, Shiroh Futaki, Tatsuto Kiwada, Yukio Sugiura
Bioorg. Med. Chem., 10(8), 2635-2639 (2002)
PMID: 12057652

Topological Stability and Self-Association of a Completely Hydrophobic Model Transmembrane Helix in Lipid Bilayers
Yoshiaki Yano, Tomokazu Takemoto, Satoe Kobayashi, Hiroyuki Yasui, Hiromu Sakurai, Wakana Ohashi, Miki Niwa, Shiroh Futaki, Yukio Sugiura, Katsumi Matsuzaki
Biochemistry, 41(9), 3073-3080 (2002)
PMID: 11863446

Artificial DNA-Bending Six-Zinc Finger Peptides with Different Charged Linkers: Distinct Kinetic Properties of DNA Bindings
Miki Imanishi, Yukio Sugiura
Biochemistry, 41(4), 1328-1334 (2002)
PMID: 11802734

Kinetic Analysis of the Interaction between Vitronectin and the Urokinase Receptor
Yuushi Okumura, Yuichi Kamikubo, Scott A. Curriden, Jieyi Wang, Tatsuto Kiwada, Shiroh Futaki, Kouki Kitagawa, David J. Loskutoff
J. Biol. Chem., 277(11), 9395-9404 (2002)
PMID: 11773078

Possible Existence of Common Internalization Mechanisms among Arginine-rich Peptides
Tomoki Suzuki, Shiroh Futaki, Miki Niwa, Seigo Tanaka, Kunihiro Ueda, Yukio Sugiura
J. Biol. Chem., 277(4), 2437-2443 (2002)
PMID: 11711547

総説類

細胞膜を透過する塩基性ペプチド−蛋白質・薬物の細胞内導入への可能性
二木史朗
蛋白質核酸酵素, 47(11), 1415-1419 (2002)

Arginine-rich Peptides: Potential for Intracellular Delivery of Macromolecules and the Mystery of the Translocation mechanisms
Shiroh Futaki
Int. J. Pharmaceutics, 245(1-2), 1-7 (2002)
PMID: 12270237